The effect of mechanical-stretch on the expression of TLR3 and myositis autoantigens in cultured C2C12 myoblasts
FENG Li-Jiang, CHEN Rong, LIU Nie-Hui, HUANG Wei-Yi, CENG Hui-Jun, LIAO Hua
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (6) : 688-691.
The effect of mechanical-stretch on the expression of TLR3 and myositis autoantigens in cultured C2C12 myoblasts
Objective To investigate the effect of mechanical-stretch on the expression of myositis autoantigens and proinflammatory TLR3 in cultured C2C12 myoblasts. Methods C2C12 cells cultured on the surface of BioFlex flexible basement membrane in vitro were subjected to cyclic strain. The proliferation of C2C12 cells was detected usingflow cytometry. The mRNA expression of myositis autoantigens (HRS, Mi-2, DNA-PKcs, U1-70) and TLR3 were analyzed through Real-time RT-PCR. Results Cyclic echanical-stretch about 2d stimulated C2C12 cell cycling and early down-regulation of mRNA levels of TLR3 and Mi-2, HRS, DNA-PKcs and U1-70 under the strain of 10% lengthening, 0.25Hz frequencyfor 2h per day(P<0.05). Conclusions Mechanical stretch reduces expression of muscle autoantigens and of pro-inflammatory TLR3, which may provide new insight to understand howresistance exercise reduce the symptoms associated with myositis.
Stretch / C2C12 / Autoantigen / TLR3
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