Effects of heroin withdrawal and relapse on the expression of TH and DβH in the neurons of VTA and NAc regions of rat brain
LI Yi-Xin, LIANG Wen-Mei, HONG Yan, JIA Bai-Juan, ZHANG Pu
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (5) : 547-550.
Effects of heroin withdrawal and relapse on the expression of TH and DβH in the neurons of VTA and NAc regions of rat brain
Objective To investigate the effect of the heroin withdrawal and relapse on the expression of TH and DβH in the ventral tegmental area(VTA) and nucleus accumbens(NAc) regions, and explore the adjustment mechanism and the relationship between dopamine(DA) and norepinephrine(NE). Methods 63 adult, male SD rats were randomly divided into three groups: normal control group (NCG), saline control group (SCG) and experiment group (EG). Experiment group was subdivided into: heroin withdrawal group (HWG), methadone detoxication group (MDG) and heroin relapse group (HRG). Brain tissues were excised after models were set up. Immunohistochemical staining was performed to test the expression of TH and DβH in the VTA and NAc regions. Results (1) TH-immunoreactive neurons expressed more TH in the VTA and NAc regions from HWG and HDG rats than that from SCG and NCG rats. After methadone detoxication, the expression of TH became normal. (2) DβH-immunoreactive neurons expressed more DβH in HWG and less DβH in MDG and HRG compared with SCG and NCG. Conclusions There is interaction between TH and DβH in the neurons of VTA and NAc regions during heroin withdrawal, methadone detoxication and heroin relapse period, and these two substances are important neurotransmitters which produce rewarding effect.
Heroin / Ventral tegmental area / Nucleus accumbens / TH / DβH
[1] McClung CA．The mechanisms of morphine addiction［J］．Rev Neurosci，2006，17( 4) : 393 -402.
[2] Martínez-Laorden E, Hurle MA, Milanés MV, et al. Morphine withdrawal activates hypothalamic-pituitary-adrenal axis and heat shock protein 27 in the left ventricle: the role of extracellular signal-regulated kinase［J］．J Pharmacol Exp Ther, 2012, 342(3):665-675.
[3] Kolik LG, Garibova TL, Litvinova SA, et al. The absence of tolerance and?withdrawal syndrome after the treatment with the new L-tryptophane-containing dipeptide anxiolytic GB-115［J］．Vestn Ross Akad Med Nauk, 2011, (7):37-42.
[4] ZachariouV, BrunzellDH, HawesJ.The neuropeptide galanin odulates behavioral and neurochemical signs of opiate withdrawal
[J]. Proc Natl Acad Sci USA, 2003,100(15):9028-9033.
[5] Kelley AE.Ventral striatal control of appetitive motivation: role in ingestive behavior and reward-related learning
[J].Neurosci Biobehav Rev, 2004, 27(8):765-776.
[6] Zheng Z, Travagli RA. Dopamine effects on identified rat vagal motoneurons
[J]. Am J Physiol Gastrointest Liver Physiol, 2007, 292(4):G1002-G1008.
[7] Haile CN, Kosten TR, Kosten TA. Pharmacogenetic treatments for drug addiction: cocaine, amphetamine and methamphetamine
[J]. Am J Drug Alcohol Abuse, 2009, 35(3):161-177.
[8] 王宇晶，牛晨，陈远寿,等.海洛因依赖大鼠VTA 区NMDA 受体介导的多巴胺神经元膜电流的变化
[J].神经解剖学杂志，2012，28( 4) : 385-391.
[9] 李一欣，梁文妹.海洛因依赖对大鼠中脑腹侧被盖区和伏隔核内DβH和CCK表达的影响
[J].神经解剖学杂志，2013，29（2）:199-203.
[10]Maldonado R, Negus S, Koob GF. Precipitation of morphine withdrawal syndrome in rats by administration of mu-, delta- and kappa-selective opioid antagonists
[J]. Neuropharmacology, 1992, 31(12):1231-1241.
[11]叶峻，蒙子卿，韦世秀,等.海洛因成瘾复吸动物模型的建立
[J].广西医科大学学报，2003, 20(6)：832-835.
[12]Paxinos G, Watson C. The rat brain in stereotaxic coordinates
[M]. 3rd edition, San Diego: Academic Press,1997:21-23.
[13]Comings DE, Blum K. Reward deficiency syndrome: genetic aspects of behavioral disorders
[J]. Prog Brain Res, 2000,126(20):325-341.
[14]Camí J, Farré M. Drug addiction
[J]. N Engl J Med, 2003, 349(10):975-986.
[15]Minoru N, Hideaki K, KanM, et al. Treatment for psychological dependence on morphine: Usefulness of inhibiting NMDA receptor and its associated protein kinase in the nucleus accumbens
[J]. Life Sci, 2005, 77(18): 2207-2220.
[16]Fitzgerald PJ. Neurodrinking: is alcohol a substrate in a novel, endogenous synthetic pathway for norepinephrine
[J]. Med Hypotheses, 2012 ,78(6):760-762.
[17]Gaval-Cruz M, Weinshenker D. Mechanisms of disulfiram-induced cocaine abstinence: antabuse and cocaine relapse
[J].Mol Interv, 2009, 9(4):175-187.
[18]Yong Z, Yan L, Zhou P, et al. Effects of thienorphine on release of dopamine and noradrenalin: an in vivo microdialysis study in rats
[J]. Neurosci Lett,?2012, 513(2):132-136.
[19]Olson VG, Heusner CL, Bland RJ, et al. Role of noradrenergic signaling by the nucleus tractus solitarius in mediating opiate reward
[J]. Science, 2006, 311(5763):1017-1120.
[20]Gómez-Milanés I, Almela P, García-Carmona JA, et al. Accumbal dopamine, noradrenaline and serotonin activity after naloxone- conditioned place aversion in morphine-dependent mice
[J]. Neurochem Int, 2012, 61(3):433-440.
[21]Van Bockstaele EJ, Qian Y, Sterling RC, et al. Low dose naltrexone administration in morphine dependent rats attenuates withdrawal-induced norepinephrineefflux in forebrain
[J].Prog Neuropsychopharmacol Biol Psychiatry, 2008, 32(4):1048-1056.
[22]Fuertes G, Laorden ML, Milanés MV. Noradrenergic and dopaminergic activity in the hypothalamic paraventricular nucleus after naloxone-inducedmorphine withdrawal
[J]. Neuroendocrinology, 2000, 71(1):60-67.
[23]Erb S.Evaluation of the relationship between anxiety during withdrawal and stress-induced reinstatement of cocaine seeking
[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2010,34(5):798-807.
[24] Tramullasé M, Martínez-Cué C, Hurlé MA. Chronic methadone treatment and repeated withdrawal impair cognition and increase the expression of apoptosisrelated proteins in mouse brain
[J]. Psychopharmacology,2007,193(1):107-120.
[25]Devoto P, Flore G, Pira L, et al. Co-release of noradrenaline and dopamine in the prefrontal cortex after acute morphine and during morphinewithdrawal
[J].Psychopharmacology(Berl), 2002,160(2):220- 224.
[26]Sofuoglu M, Kosten TR. Emerging pharmacological strategies in the fight against cocaine addiction
[J].Expert Opin Emerg Drugs, 2006,11(1):91-98.
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