Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease
CAO Jian, WANG Rui, WANG Qian, WANG Shuang
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (4) : 453-456.
Effects of vitamin C on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's Disease
Objective To investigate the effect and the related mechanism of Vitamin C (Vit C) on PC12 cells apoptosis in MPP+ induced cell model of Parkinson's disease (PD). Methods PC12 cells were divided to MPP+ induced cell model of PD group (MPP+ group) and Vit C treatment group (Vit C group). Firstly, Vit C group was incubated with 250 μmol/L Vit C 6 h, and then the two groups were treated with 100 μmol/L MPP+ 12 h. The LDH concentration was measured by LDH kit. The TUNEL method was performed to measure PC12 cells apoptosis in two groups. The expression of Caspase3 and c-Caspase3 was detected by Western-blot. Results Compared with MPP+ group, Vit C significantly inhibited LDH concentration and decreased apoptosis of PC12 cells, indicating that Vit C could down-regulate the expression of c-Caspase3 (P<0.05). Conclusion Vit C can decrease apoptosis of PC12 cells in vitro significantlyand play important protection role in PD via down-regulation of the expression of c-Caspase3
Parkinson's disease / TUNEL method / Apoptosis / Vitamin C / MPP+
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