Experimental study on bone formation potential of the intervening tissue at the hypertrophic nonunion site
LIU Lian-Song, XU Jia, KANG Qiang-Lin, XUE Feng, ZHANG Chang-Jing, CHAI Yi-Min
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (4) : 441-446.
Experimental study on bone formation potential of the intervening tissue at the hypertrophic nonunion site
Objective To observe the structure of the intervening and surrounding tissue at the hypertrophic nonunion site, to assess the potential of bone formation. Methods Tissue from the nonunion site and its surroundings was extracted from 25 patients with hypertrophic nonunion. Tissue structure and microscopic characteristics of these regions were investigated by optical microscope, transmission electron microscopy. Expression of BMP-2 was detected by immunohistochemistry. The positive cells were observed, counted and analyzed statistically. Results The area of hypertrophic nonunion ends displayed different non-uniform histological features. The center area mainly displayed fibrotic tissue which contained a large number of fibroblast-like cells; the marginal area contained masses of chondrocytes without neovascularization; the scar area contained a certain amount of osteoblasts with rich neovascularization. The results of immunohistochemistry showed the expression of BMP-2 was more intense in the callus area than in the center area or in the marginal area(P<0.05). Conclusion Different area of hypertrophic nonunion ends displays different histological features and cytological characteristics. The decreasing of BMP-2 in the nonunion ends results in different potential of bone formation.
Hypertrophic nonunion / Bone ends / Callus / Potential of bone formation / BMP-2
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