Dynamic expression characteristics of Notch signal in bone marrow-derived mesenchymal stem cells during the process of differentiation into hepatocytes
LIU Ke, LIU Gui-Yang, YANG Xiang, TUN Zhang-Lin, HUANG Wen-Hua
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (3) : 308-313.
Dynamic expression characteristics of Notch signal in bone marrow-derived mesenchymal stem cells during the process of differentiation into hepatocytes
Objective To explore the effect of Notch signaling in bone marrow-derived mesenchymal stem cells during the process of differentiation into hepatocytes. Methods An induction system under which BM-MSCs can differentiate into hepatocytes was established. On days 0, 7, 11 and 21 day during the differentiation direction, nine key genes in Notch pathway were selected as target genes and determined in BM-MSCs using technique reverse dot blot hybridization. The results of reverse dot blot hybridization showed that the effect of Notch signaling in BM-MSCs during the process of differentiation into hepatocytes. At the same time，the results of RT-PCR analysis reveals the differentiation status of BM-MSCs. Results On the 21th day when the differentiation direction was determined in BM-MSCs, the mRNA level of nine key genes was significantly lower than that on days 0, 7, and 11. In the further experiments, down-regulation of Notch signaling was shown to be critical for BM-MSCs to differentiate into hepatocytes, as increased Jagged1 resulted in up-regulated Notch activation, leading to higher levels of expression of Hes1 and Hey1, which completely blocked Albumin expresion in BM-MSCs. Conclusion These results in our study showed that Notch signaling in BM-MSCs was necessary to initiate differentiation into hepatocytes, but must be down-regulated for the differentiation to proceed continuously.
Adult stem cell / Mesenchymal stem cell / Directing-differentiation / Hepatocyte / Notch
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