Study on the molecular mechanism of mechanical compression treating hyperplastic scar via TGF-β 1 pathway
SHEN Kuan-Hong, HUANG Dong, TUN Wei-Chi, HUANG Yong-Jun, MAO Yong
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (3) : 299-302.
Study on the molecular mechanism of mechanical compression treating hyperplastic scar via TGF-β 1 pathway
Objective To study the effects of mechanical compression on the expression of TGF-β1 receptor，smads and collagen from human skin hypertrophic scar fibroblast via the mechanical pressure system intervention, and explore the potential molecular mechanism of pressure treatment of hyperplastic scar. Methods Hypertrophic scar cells were cultured in vitro ，the expression of TGF-β1 receptor，Smad3,Smad7 and collagens were determined on mRNA level by RT-PCR; Difference in expression between the mechanical pressure intervention experimental group and the control group in the hypertrophic scar fibroblasts was tested. Results The RT - PCR results indicated that the expression of smad3 and smad7, Collagen Ⅰ was reduced after pressure treatment the difference being statistically significant (P<0.05), whereas the expression of TGF-β1 receptor increased, the difference being statistically significant (P<0.05). Conclusion Mechanical compression can inhibit Smad3 expression and promote TGF-β1 receptor expression so as to promote the degradation of hyperplastic scar collagen.
Hypertrophic scar / Fibroblast / Mechanical compression / TGF-β / Collagen
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