Impact of hair growth phase on mouse skin wound healing
KONG E-Nan, ZHANG Min, CUI Meng-Qing, LI Liang-Beng, ZHOU Zhi-Chao, ZHANG Lin
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (3) : 283-287.
Impact of hair growth phase on mouse skin wound healing
Objective To investigate the skin wound healing speed of C57BL/6 mice at various stages of hair growth cycle. Methods Mouse skin wounding models were established and wound healing rate in the following manner was calculated: Wound closure rate (%) = [(Original wound area - Open area on the final day)/Original wound area] ×100%. Wound healing speed of mouse at hair anagen stages and at hair telogen stages was compared. H&E staining was adopted to compare differences in morphological and histological characteristics of wound healing. BrdU was used to detect cell proliferation around wounds. Results Wound healing rate of mouse at hair anagen stages was significant higher than that of mouse at telogen stages. H&E staining showed that mice presented relatively more epidermal cell layers around wounds and epidermal cell layers migrated faster at hair anagen stages. BrdU detection showed that mice at hair anagen stages had a larger number of BrdU+ epidermal cells around wounds than mouse at telogen stages. Conclusion Mice at hair anagen stages enjoy a faster skin wound healing rate than mice at telogen stages, which provides a basis for further research into the role of hair follicle in wound healing process as well as a guidance on how to choose animal models for study of skin wound healing.
[1] 罗满林.实验动物学
[M].北京：中国农业出版社, 2002：87-90.
[2] 张敏,朱晓文,张琳等. C57BL/6小鼠皮肤毛囊发育的研究
[J]. 中国临床解剖学杂志, 2010, 28(1):74-77.
[3] 孙丽, 张敏, 张琳，等. Activin B联合BMSC不同移植方式对大鼠皮肤创伤愈合的治疗
[J]. 中国临床解剖学杂志, 2012, 30(2):197-202.
[4] Müller-R?ver SB, Handjiski C, van der Veen S, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages
[J]. J Invest Dermatol, 2001, 117(1): 3-15.
[5] Zhang M, Liu NY, Wang XE, et al. Activin B promotes epithelial wound healing in vivo through RhoA-JNK signaling pathway
[J]. PLoS One, 2011, 6(9):e25143.
[6] 杨宗成.烧伤治疗学
[M].北京：人民卫生出版社, 2006：180-181.
[7] 邹仲之.组织学与胚胎学
[M]. 北京：人民卫生出版社, 2004：115-116.
[8] Lavker RM, Sun TT, Oshima H, et al. Hair follicle stem cells
[J]. Investig Dermatol Symp Proc, 2003, 8(1):28-38.
[9] Plikus MV, Gay DL, Treffeisen E, et al. Epithelial stem cells and implications for wound repair
[J]. Semin Cell Dev Biol, 2012, 23(9):946-953.
[10] Brownell I, Guevara E, Bai CB, et al. Nerve-derived sonichedgehog defines a niche for hair follicle stem cells capable of becoming epidermal stem cells
[J]. Cell Stem Cell, 2011, 8(5):552-565.
[11] Tiede S, Kloepper JE, Bodò E, et al. Hair follicle stem cells: walking the maze
[J]. Eur J Cell Biol, 2007, 86(7):355-376.
[12] Ito M, Cotsarelis G. Is the hair follicle necessary for normal wound healing
[J]. Invest Dermatol, 2008, 128(5):1059-1061.
[13] Qi SH, Liu P, Xie JL, et al. Experimental study on repairing of nude mice skin defects with composite skin consisting of xenogeneic dermis and epidermal stem cells and hair follicle dermal papilla cells
[J]. Burns, 2008, 34(3):385-392.
/
| 〈 |
|
〉 |
