Effects of acute hypoxia on TH expression in sympathetic ganglion of WKY rats
HUI Chu-Ping, XU Jian, CHAN Na-Na, LIU Wei, WANG Yong-Kuo, YANG De-Hui, CHAN Yun-Guan, XU Feng-Xiao
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (2) : 188-191.
Effects of acute hypoxia on TH expression in sympathetic ganglion of WKY rats
Objective To discuss the effects of tyrosine hydroxylase (TH) in the occurrence and development of high altitude hypertension by observing the changes of the ultramicrostructure of the sympathetic nerve cells and the expression TH in superior cervical ganglion of rats. Methods WKY rats were randomly divided to be raised at plain or in the altitude chambers imitating the environment at 5000 m for 1 d, 3 d and 7 d, respectively. Electron microscopy was used to examine the changes of the ultramicrostructure of the sympathetic nerve cells. Real-time PCR and Western blot method were used to examine the mRNA and protein expression of TH. Results The blood pressure of hypoxia rats was obviously higher than C group（P<0.05）;the results of Real-time PCR and Western blot showed that mRNA and protein expression of TH in superior cervical ganglion of H1 and H3 groups were lower than C group（P<0.05）, but the expression in H7 group were higher than C group. The results of electron microscopy showed that the morphology of sympathetic nerve cells, quantity and structure of cell organelle were changed in the circumstance of plateau. Conclusions TH may participate in the occurrence of high altitude hypertension through certain complex mechanisms.
Plateau / Hypoxia / Sympathetic ganglion / TH
[1] Parrish DC, Gritman K, Van Winkle DM, et al. Postinfarct sympathetic hyperactivity differentially stimulates expression of tyrosine hydroxylase and norepinephrine transporter
[J]. Am J Physiol Heart Circ Physiol,2008,294(1): H99-H106.
[2] Gozal E, Shah ZA, Pequignot JM, et al. Tyrosine hydroxylase expression and activity in the rat brain: differential regulation after long-term intermittent or sustained hypoxia
[J]. J Appl Physiol,2005,99(2):642-649.
[3] Huh JW, Kim SY, Lee JH, et al.YC-1 attenuates hypoxia-induced pulmonary arterial hypertension in mice
[J]. Pulm Pharmacol Ther,2011,24(6): 638-646.
[4] Pasha MA, Newman JH. High-altitude disorders: pulmonary hypertension: pulmonary vascular disease: the global perspective
[J]. Chest,2010,137(6 suppl):13S-19S.
[5] Alves FH, Crestani CC, Resstel LB. Cardiovascular effects of noradrenaline microinjected into the insular cortex of unanesthetized rats
[J]. Auton Neurosci, 2011,160(1-2):90-98.
[6] Dorn GW 2nd. Adrenergic signaling polymorphisms and their impact on cardiovascular disease
[J].Physiol Rev, 2010, 90(3):1013-1062.
[7] Yagi K, Miki K. Renal sympathetic nerve activity plays a critical role in inducing hypertension by cold exposure
[J]. Neurosci Res,2011,71(9):e161.
[8] Grassi G, Cattaneo BM, Seravalle G,et al. Baroreflex control of sympathetic nerve activity in essential and secondary hypertension
[J]. Hypertension,1998,31(1):68-72.
/
| 〈 |
|
〉 |
