Protection and mechanism of tetrahydroxy stilbene glucoside on hippocampal ischemia-reperfusion injury in gerbils
WANG Ji, CHEN Xiao-Yu, LIU Mei-Mei, WANG Dong, SUN Yu-Hua, CHEN Xiao-Rong
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (2) : 180-183.
Protection and mechanism of tetrahydroxy stilbene glucoside on hippocampal ischemia-reperfusion injury in gerbils
Objective To investigate the protective effects of tetrahydroxy stilbene glucoside (TSG) on ischemia-reperfusion (I/R)-induced hippocampal injury and the underlying mechanisms in gerbils. Methods Gerbil global I/R model was established by clamping bilateral carotid arteries for 30 min followed by reperfusion for 5 days. Animals were divided into 6 groups randomly: Sham, I/R, I/R+TSG high, middle, low (6, 3, 1.5 mg/kg) and edaravone injection group (3 mg/kg). The functions of learning and memory were detected by Morris water maze 5 days after reperfusion. The number and structure of neurons and neuronal apoptosis in hippocampal CA1 region were evaluated by Nissl and TUNEL staining, respectively. In addition, the expression of active-caspase-3 in cerebral tissues was measured by Western blot. Results Compared to Sham group, learning and memory capacity of gerbils in I/R group declined significantly, the number of functional neurons in hippocampal CA1 region decreased dramatically. Furthermore, the apoptotic neurons indicated by TUNEL staining as well as caspase-3 activation increased remarkably. TSG administration at middle and high dose (3, 6 mg/kg) and edaravone injection significantly reversed aforementioned changes induced by I/R. In contrast, TSG administration at low dose (1.5 mg/kg) had no effect. Conclusion TSG had therapeutic effects on I/R induced cerebral injury, especially on delayed neuron apoptosis in hippocampal CA1 region, which may be related to its suppression effect on caspase-3 activation.
Ischemia-reperfusion / Tetrahydroxy stilbene glucoside / Hippocampal neuron / Apoptosis
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