Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats
QI Wei, SUN Shan-Quan, DAN Jian-Hua, HUANG Sai-Qin, WU Xiu-Yu, HOU Liang-Juan, ZHUO Fei, CHEN Zhen
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (1) : 81-84.
Endogenous proliferation and morphological characteristics of NG2-cells in compressed spinal cord injury of rats
Objective To investigate endogenous proliferation and morphological characteristics of NG2-cells in the white matter of compressed spinal cord injury (ACSCI) of rats. Methods 42 adult SD rats were randomly divided into 2 groups: model group (n=21), which underwent spinal cord compression by a self-made device, and sham group (n=21), which underwent only laminectomy. Expression of NG2-positive cells was detected by IHC after 1, 3 and 7 days. The average number, soma area, process length of NG2+-cells were measured by Image Pro Plus6.0. Results Compared with control group (19.92±6.68 per field), the number of NG2+-cells increased at 1d (30.17±11.08 per field), peaked at 3d (90.75±9.40 per field), and decreased at 7d (78.38±8.91 per field) (P＜0.05). NG2+-cells' soma became smaller (128.25±32.06 μm2) and processes became shorter (10.98±4.25 μm) at 1d, then NG2+-cells' soma became larger (225.26±16.64 μm2) and processes became longer (18.63±2.26 μm) at 3d (P＜0.05). However, the soma area and process length of NG2+-cells at 7d kept unchanged compared with that at 3d (P＞0.05). There were many clusters of NG2+ cells that had smaller and round soma, fewer or no processes after CSCI. Conclusion NG2+-cells could proliferate actively with the soma growing larger and the process growing longer but still shorter than normal in CSCI after a week.
Compressed spinal cord injury / / NG2 cells / / Endogenous proliferation / / Cellular morphology
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