Acquisition of digital dataset of celloidin axial sectional images of temporal bone and comparative observation with CT images
LI Xi-Beng, JIA Yin
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (1) : 51-55.
Acquisition of digital dataset of celloidin axial sectional images of temporal bone and comparative observation with CT images
Objective To make sequential axial sectional slices of the lateral skull base and obtain digitized dataset of the lateral skull base in order to provide reference for sectional study of imaging and clinical diagnosis of lateral skull base diseases. Methods Two cadaveric heads underwent coronal section after spiral CT scanning. After celloidin embedding, a brain slicer was used to make axial thin slices (100 μm), which was then photographed with a Sony F-717 and saved in a computer representative sections were selected for HE staining. Then the digital photo, the HE staining picture and the CT image were compared to describe the characteristics of anatomical structures, including the common crus plane, lateral semicircular canal plane, the horizontal segment of facial nerve plane, the vestibular window plane, the round window plane, internal orifice of jugular foramen plane. Results Two sets of complete axial digital thin sectional data were obtained (300 and 340 images, respectively). Delicate structures were shown clearly and spatial relationships between anatomical structures can be understood deeply by sequential, histological and CT comparative observation. Conclusion Celloidin embedding is an important method for obtaining high resolution sequential digital imaging data of lateral skull base. Histological and CT image comparative observation is an ideal method for studying characteristics of sectional anatomy of the lateral skull base. It provides valuble reference for imaging diagnosis.
Skull base, Lateral / Celloidin / Anatomy / ,Section / Axial
[1] Parlier CC, Champsaur P, Perrin E，et al. High-resolution computed tomography of the canals of the temporal bone : anatomic correlations
[J]. Surg Radiol Anat, 1998, 20(6) :437-444.
[2] 靳颖, 刘津平, 田德润, 等. 颞骨的断面解剖学研究及其临床意义
[J]. 中国临床解剖学杂志, 2004, 22(4):353-356.
[3] 杨开明．医学影像学的发展与断层解剖学的未来
[J]．实用医学影像杂志，2002，3(4)：295-296
[4] Hayran M, Onerci M, Ozturk C.Evaluation of the temporal bone by anatomic sections and computed tomography
[J]. Surg Radiol Anat,1992,14(2):169-173.
[5] Harada T, Ishii S, Tayama N.Three-dimensional reconstruction of the temporal bone from histologic sections
[J].Arch Otolaryngol Head Neck Surg,1988,114(10):1139-1142.
[6] Dai P, Liu Y, Jiang SC, et al. Stereo morphology of temporal bone and ear
[J].Chin Med J (Engl), 2004, 117(5):733-737.
[7] Qiu MG. Zhang SX, Liu ZJ, et al. Plastination and computerized 3D reconstruction of the temporal bone
[J]. Clin Anat, 2003, 16(4):300-303.
[8] 李希平，夏寅，韩德民. 基于虚拟中国人数据集的鼻部及颞骨解剖结构三维重建
[J].中国临床解剖学杂志, 2004, 22(4)：377-379.
[9] S?rensen MS, Dobrzeniecki AB, Larsen P, et al. The visible ear: a digital image library of the temporal bone
[J]. ORL J Otorhinolaryngol Relat Spec, 2002 ,64(6):378-381.
[10]李希平，韩德民，夏寅.基于颞骨冰冻切片数据集的侧颅底区解剖结构三维重建
[J].中国耳鼻咽喉头颈外科, 2007, 14(3)：149-151.
[11]Wang H, Merchant SN,?Sorensen?MS. A downloadable three-dimensional virtual model of the visible ear
[J].ORL J Otorhinolaryngol Relat Spec,2007,69(2):63-67.
[12]Cooper MH, Archer CR, Kveton JF. Correlation of high-resolution computed tomography and gross anatomic sections of the temporal bone:part I.The facial nerve
[J]. Am J Otol,1987,8(5):375-384.
[13]Cooper MH, Archer CR, Kveton JF.Correlation of high resolution computed tomography and gross anatomic sections of the temporal bone. Part III. Cochlear and vestibular aqueducts
[J].Am J Otol,1989,10(4):272-276.
[14]Archer CR, Cooper MH, Kveton JF. Correlation of high-resolution computed tomography and gross anatomic sections of the temporal bone:II.Vestibular apparatus
[J]. Am J Otol, 1988, 9(4):276-281.
[15]段菊如，林敏，熊俊平．面神经颞骨内段在横断薄层和CT上的定位及临床意义
[J]. 中国临床解剖学杂志, 2004, 22(3):257-260.
[16]段菊如，罗红强，鲁纯纠, 等.圆窗区区颞骨切片和CT对照观察
[J].中华耳鼻咽喉科杂志, 2004, 39(5):269-272.
/
| 〈 |
|
〉 |
