The role of PGE2 and NF-κB pathway during the bone regeneration
QIU Xiao-Zhong, WANG Guo-Bao, HU Xiao-Fang, WANG Yong-Kuai, ZHANG Ma-Hui, WANG Le-Yu
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (3) : 315-319.
The role of PGE2 and NF-κB pathway during the bone regeneration
Objective To study the relationship between the PGE2 production and NF-κB activation in bone regeneration, by combined analysis of the PGE2 production and NF-κB activation in the MC3T3-E1 cells and rat fracture model. Methods PGE2 and NF-κB inhibitor BAY 11-708246 were used to deal with MC3T3-E1 cells and rat fracture model. PGE2 production, NF-κB activation and the expression of BMP-7, Id2 and SOD2 were detected during the bone regeneration adopting ALP activity assay, western blot, EMSA analysis and ELISA assay. Results After being challenged with 10umol/l PGE2 for 10 min, 30 min and 2h respectively, both of the cell proliferation and differentiation of MC3T3- E1 cells were induced. However, 5umol/l BAY 11-7082 can inhibit the effects of PGE2. This implied that PGE2 induced the expression of ALP through the NF-κB pathway. Local treatment of BAY 11-7082, the production of PGE2 was decreased. On the other hand, Higher NF-κB activation, higher expression of BMP-7and SOD2, and the lower expression of Id2 were appeared in the site of bone fracture. After being injecting of BAY 11-7082, the expression of BMP-7 and Id2 were restored, while the expression of SOD2 was kept unchanged. Conclusions PGE2 can inhibit the expression of Id2 and the activation of NF-κB at the same time during the bone regeneration.
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