Expression of eNOS and iNOS in postnatal rat ovaries at different developmental days
JUE Li-Hua, JUE Jiao-Hua, LONG Chi-Feng, LI Mei-Xiang, DAN Jin-Feng, LUO Gong-Mei, MO Zhong-Cheng
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (2) : 203-208.
Expression of eNOS and iNOS in postnatal rat ovaries at different developmental days
Objective To observe the expression of eNOS and iNOS in varies and investigate its significance. Methods Immunohistochemical technology and image analysis system were utilized to detect the expression of eNOS and iNOS in ovaries from postnatal rat at different developmental days. Results Positive reaction for eNOS and iNOS was weak in oocytes of rat ovaries at day 0. In ovaries from older rat than day 4, both of eNOS and iNOS immunoreactivity were intense in health oocytes and very weak in degenerative oocytes. Expression of iNOS was strong positive in follicular cells and theca cells of ovaries from younger rat than day 30，but became very weak in those of growing follicles from older rat than day 30, and increased in those of atretic follicles compared with those in health growing follicles. Positive expression of eNOS was constant. Interstitium and atretic follicles increased in ovaries from older rat than day 240, and interstitium showed an enhanced expression for iNOS. Ovaries from older rat than day 360 became fibrosis, in which iNOS stain was more intensive than eNOS. Conclusion In rat ovaries from different development stages, expression of eNOS is relatively stable and expression of iNOS is the minimum in child-bearing period after sex maturation, but increases gradually with rat age (month) progressing and ovarian functional deterioration.
Ovary / eNOS / iNOS / Immunohistochemistry / Rat
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