The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene
QIU Jia-Qi, ZHANG Ming-Sheng, LI Xin-Beng, BAI Wen-Fang, TUN Bo, BAI Li-Meng, XU Wu-Hua
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (1) : 74-78.
The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene
Objective To explore the effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by Brain-derived neurotrophic factor(BDNF) gene. Methods The replication-deficient recombinant adenoviruses containing BDNF gene（rADBDNF）was structured by the pAd-easy I adenovirus packaging systems in vitro. Rat BMSCs were isolated from rat femoral and tibial bone marrow, cultured and identified. BMSCs infected with rADBDNF were exposed to low frequency electromagnetic fields. The proliferation capacity of BMSCs was analyzed using the trypan blue staining assay and MTT assay, and cell cycles of BMSCs was evaluated by flow cytometry. Results BMSCs modified by rADBDNF had no significant change on cell proliferation compared with that of untreated cells (P>0.05). However, electromagnetic field exposed MSCs(LFEMF group) had a significant proliferative improvement compared with the control(P<0.05). The ratio of G2/M cell of the transgenic and LFEMF group was also increased. Conclusions LFEMF could enhance the cell proliferation of rat BMSCs modified by BDNF gene.
Bone mesenchymal stem cells(BMSCs) / Cell proliferation / Brain-derived neurotrophic factor(BDNF) / Low frequency electromagnetic fields(LFEMF)
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