The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold
CHEN Pan, XU Bo, LI Song-Jian, CHEN Zhong, LIU Cheng-Long
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (6) : 686-689.
The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold
Objective To evaluate the biocompatibility of an injectable chitosan (CS) /nano-hydroxyapatite(HA)/collagen (Col) scaffold. Methods rBMSCs was mixed with CS/HA/Col solution and subcutaneously injected into Wistar rats. After 24h, 14d and 28d, the animals were sacrificed and subcutaneous tissues at injecting site were removed. HE, MASSON and immunohistochemical stainings were performed for evaluating the features if scaffolds. Results The liquid CS/HA/Col (room temperature) would form the gel after injecting into the subcutaneous tissue. The mixed rBMSCs survived well in the CS/HA/Col gel at even 28 days in vivo. The CS/HA/Col/rBMSCs gel induced less inflammatory reaction in the host tissue compared to that of simple CS/HA/Col gel. Conclusions Our results suggest that the CS/HA/Col system can be used to load rBMSCs in vitro homogeneously. The CS/HA/Col system can be used in vivo with the minimally invasive manner and feasible biocompatible environment.
[1] Zhang W, Liao SS, Cui FZ. Hierarchical self-assembly of nano-fibrils in mineralized collagen
[J]. Chemistry of Materials,2003, 15(16):3221–3226.
[2] Huang Z, Feng QL, Yu B, et al. Biomimetic properties of an injectable chitosan/nano -hydroxyapatite/collagen composite
[J].Materials Science and Engineering,2011,31(3):683–687.
[3]Porter JR, Ruckh TT, Popat KC. Bone tissue engineering: a review in bone biomimetics and drug delivery strategies
[J]. Biotechnol Prog, 2009, 25(6): 1539-1560.
[4]Di Martino A, Sittinger M, Risbud MV. Chitosan：a versatile biopolymer for orthopaedic tissue-engineering
[J]. Biomaterials,2005, 26(30):5983-5990.
[5]Hoemann CD, Sun J, Legare A, et al. Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle
[J]. Osteoarthritis Cartilage, 2005, 13(4): 318-329.
[6] Ma GP, Yang DZ, Li QZ, et al. Injectable hydrogels based on chitosan derivative/polyethylene glycol dimethacrylate/N, N-dimethyla crylamide as bone tissue engineering matrix
[J]. Carbohydrate Polymers, 2009,79(3):620–627.
[7] Muzzarelli RAA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone
[J]. Carbohydrate Polymers, 2009, 76(2):167–182.
[8]Bartholomew A, Sturgeon C, Siatskas M, et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo
[J]. Experimental Hematology, 2002,30(1): 42–48.
[9]Di Nicola M, Carlo-Stella C, Magni M, et al. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellularor nonspecific mitogenic stimuli
[J]. Blood, 2002,99(10):3838–3843.
[10]Djouad F, Plence P, Bony C, et al. Immunosuppressiveeffect of mesenchymal stem cells favors tumor growth in allogeneic animals
[J]. Blood, 2003,102(10):3837-3844.
[11]Gratzner HG. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine:A new reagent for detection of DNA replication
[J]. Science,1982, 218(4571): 474-475.
[12]Sasaki T, Kitagawa K, Omura-Matsuoka E, et al. The phosphodiesterase inhibitor rolipram promotesSurvival of newborn hippocampal neurons after ischemia
[J]. Stroke, 2007, 38(5): 1597-1605.
[13]Zhao LR, Seema S, Duan WM, et al. Brain repair by hematopoietic growth factors in a rat model of stroke
[J]. Stroke, 2007, 38(9): 2584-2591.
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