Characterization and biocompatible evaluation of nHA／RHLC／PLA composite scaffold
LV Yu-Meng, CHENG Li-Meng, FEI Guo-Xian, WANG Yu, CUI Fu-Zhai
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (5) : 550-556.
Characterization and biocompatible evaluation of nHA／RHLC／PLA composite scaffold
Objective To explore the characteristics and biocompatibility of nano-hydroxyapatite/ recombinant human-like collagen/polylactic acid (nHA/RHLC/PLA ) composite scaffold. Methods The morphologic features of nHA/RHLC/PLA scaffold were observed under scanning electron microscopy．The porosity of the scaffold was determined via isopropyl alcohol displacement method. The canine BMSCs were separated and cultured in vitro. The third passage of cells was seeded into the scaffold for analyzing the adhesion and proliferation ability of BMSCs on scaffold. Results Scan electron microscopy showed that, the surface of scaffold was porous．BMSCs infiltrated into the pores of scaffold, adhered, spread, and proliferated on the surface of scaffold pore within one week. However, we did not detected the significant difference of adhesion and proliferation of BMSCs between nHA/RHLC/PLA scaffold culture and normal culture (P>0.05). Conclusions The nHA/RHLC/PLA composite scaffold has favourable microstructure, which is biocompatible to BMSCs.
Nanocomposite / Stem cells / Tissue engineering / / Biocompatibility / Cell culture
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