The effect of the self-assemble peptide nanofiber scaffold on the proliferation and differentiation of MCSCs in vitro
CUI Xiao-Jun, TAN Yu-Zhen, LI Shao-Hua, GUO Hai-Dong, WANG Cun, ZHANG Jian-Kai
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (4) : 428-432.
The effect of the self-assemble peptide nanofiber scaffold on the proliferation and differentiation of MCSCs in vitro
Objective To explore the effect of the self-assemble peptide nanofiber scaffold (NFS) on the proliferation and differentiation of marrow-derived cardiac stem cells (MCSCs). Methods The self-assemble peptides were designed and synthesized. The self-assemble state was viewed by AFM and SEM. The compatibility of MCSCs with self-assembling peptide nanofiber scarffolds was viewed by SEM. The effect of NFS on the growth of MCSCs was detected by CCK-8. Through cardiac-specific troponin cTnT staining, the effect of NFS was evaluated on myocardial cell differentiation of MCSCs. Results SEM showed the self-assembly of the peptides into the retiform interwoven nanofiber scaffold structure, with the stent diameter of 50 ~ 200 nm. MCSCs were seeded into the self-assemble peptide nanofibers 1 day later. Cells were detected in the network-like nanofiber, which closely linked with the nano-fibers under SEM. The viability of cells in NFS was significantly higher than that of control group. The cells in NFS contacted closely with each other, and demonstrated the same direction and arrangement. After 4 weeks' induction, all of cells expressed cTnT. Conclusions NFS and MCSCs have good biocompatibility. The three-dimensional micro-environment of NFS is valuable for the growth and differentiation of MCSCs in vitro.
Self-assemble peptide nanofibers / Bone marrow-derived cardiac stem cells / Biocompatibility / Differentiation
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