Objective　To investigate the anatomic formation of the glabella muscles and provide anatomical basis for the use of Botulinum Toxin Injection.  Methods　6 adult cephalic specimens were anatomized. The landmarks of the glabella muscles, including the corrugator, the depressor supercilii and the procerus were measured. The weights and cross-sectional area of the muscle were also examined.　Results　The origin of the glabella muscles is constant, while the insertion is an area which is hard to define. Moreover, each pathetic muscle is connected closely to form a "combination complex", especially between the depressor supercilii and the procerus. The appearance of the glabellar wrinkle is formed by horizontal force generated through contraction of the corrugators and the depressor supercilii. The proportion of the physiological cross-sectional area is 6:1 between the corrugator and the depressor supercilii. The proportion of the clinical anatomy horizontal force is（8-10）:1 between the corrugator and the depressor supercilii.　Conclusions　The corrugators and the depressor supercilii are the potent targets for Botulinum Toxin Injection. Injection points was selected at originating 1/3 of the corrugator point and depressor supercilii combining parts .