Experimental study on revascularization detection in tissue engineered scaffold using vascular cast technique
WU Yan-Ge, YANG Lin, SUN Xua-Feng, WANG Zheng
Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (1) : 87-88.
Experimental study on revascularization detection in tissue engineered scaffold using vascular cast technique
Objective To explore the feasibility of vascular cast for revascularization of tissue engineered organ. Method Tissue engineered scaffolds DegraPol were implanted in rat omental 1 weeks. The anatomic technology of vascular cast was used to observe revascularization of DegraPol scaffold. Results Vascular cast showed more blue vessels around the DegraPol scaffold in omental implants. Micro-vessels were found grew into the porous scaffold. Conclusions Vascular cast technique is suitable for detecing the microvascular, and could be used as a new method to observe revascularization of tissue engineered organ.
Vascular cast / Tissue-engineering / Scaffold / Revascularization
[1] Griffith LG, Naughton G. Tissue engineering-current challenges and expanding opportunities
[J]. Science, 2002, 295(8): 1009-1014.
[2] 张 晔, 曾秉芳. 组织工程与血管形成
[J]. 国外医学骨科分册, 2003, 24(6): 363-367.
[3] 曾宪利, 裴国献. 组织工程骨血管化机理及策略的研究进展. 实用手外科杂志,2005, 19(3): 193-195.
[4] Bouhadir KH, Mooney DJ. Promoting angiogenesis in engineered tissues
[J]. J Drug Target, 2001, 9(6): 397-406.
[5] Sahota PS, Burn JL, Heaton M, et al. Development of a reconstructed human skin model for angiogenesis
[J]. Wound Repair Regen, 2003, 11(4): 275-284.
[6] Wenger A, Stahl A, Weber H, et al. Modulation of in vitro angiogenesis in a three-dimensional spheroidal coculture model for bone tissue engineering
[J]. Tissue Eng, 2004, 10(9-10): 1536-1547.
[7] Suh S, Kim J, Shin J, Kil K. Use of omentum as an in vivo cell culture system in tissue engineering
[J]. ASAIO J, 2004, 50(5): 464-467.
[8] Patrick CW Jr, Chauvin PB, Hobley J. Preadipocyte seeded PLGA scaffolds for adipose tissue engineering
[J]. Tissue Eng, 1999,5(2): 139-151.
[9] Philippe P, Franck Villars, Simone MP. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats
[J]. Plast Res Surg,2003,111(6): 1932-1941.
[10]Neumann T, Nicholson BS, Sanders JE. Tissue engineering of perfused microvessels
[J]. Microvasc Res,2003,66(1): 59-67.
[11]王学明, 裴国献. 组织工程骨血管化的监测
[J]. 中华创伤骨科, 2004, 6(7): 822-824.
[12]Ribatti D, Nico B, Vacca A, et al. Chorioallantoic membrane capillary bed: a useful target for studying angiogenesis and antiangiogenesis in vivo
[J]. Anat Rec, 2001, 264 (4):317-324.
[13]Borges J, Tegtmeier FT, Padron NT, et al. Chorioallantoic membrane angiogenesis model for tissue engineering: A new twist on a classic model
[J]. Tissue Eng, 2003,9(3):441-450.
/
| 〈 |
|
〉 |
