Analysis on the mechanism of spastic sarcomere remodeling after stroke based on omics changes of the ubiquitin proteasome system and related proteins

doi:10.13418/j.issn.1001-165x.2026.3.04

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Chinese Journal of Clinical Anatomy ›› 2026, Vol. 44 ›› Issue (3) : 265-273. DOI: 10.13418/j.issn.1001-165x.2026.3.04

Analysis on the mechanism of spastic sarcomere remodeling after stroke based on omics changes of the ubiquitin proteasome system and related proteins

Liu Guangrun, He Yun, Jiang Xiaolin, Yang Shengbo*

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Abstract

Objective To analyze the changes of the ubiquitin proteasome system and related proteins in the gastrocnemius muscle of rats with post-stroke spasm through quantitative proteomics, and to elucidate the mechanism of sarcomere remodeling. Methods Sixteen Sprague Dawley rats were divided into a normal group and a post-stroke muscle spasm group. A stroke muscle spasm model was created by using the nylon thread embolization. Neurological function damage was evaluated using the Zea Longa score. Gastrocnemius muscle tone was measured using a biological signal analyzer, and the length, number, and width of gastrocnemius muscle segments were measured by using Mallory phosphotungstic acid hematoxylin staining. The changes in the ubiquitin protease system and related proteins were quantitatively analyzed by using proteomics. Results Compared with the normal group, the Zea Longa score and gastrocnemius muscle tone were significantly increased in the post-stroke spasticity group, with an increased sarcomere length and decreased sarcomere number and decreased width, P<0.05, the difference was statistically significant. Bioinformatics analysis revealed that the expression of Ube2b and Ube2n, Trim63 and Fbxo32, as well as 32 proteasome subunits, were upregulated in the post-stroke muscle spasm group, while their related proteins Myh1 and Des were significantly downregulated. Conclusions After stroke, the muscle remodeling in the spastic gastrocnemius muscle of rats may be due to the upregulation of the ubiquitin proteasome system leading to increased hydrolysis of Myh1 and desmin.

Key words

Stroke / / / Muscle spasms / / / Ubiquitin proteasome system / / / Sarcomere remodeling / / / Proteomic analysis

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Liu Guangrun, He Yun, Jiang Xiaolin, Yang Shengbo. Analysis on the mechanism of spastic sarcomere remodeling after stroke based on omics changes of the ubiquitin proteasome system and related proteins[J]. Chinese Journal of Clinical Anatomy. 2026, 44(3): 265-273 https://doi.org/10.13418/j.issn.1001-165x.2026.3.04

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