Objective To locate of position of the ventricle system by rat ventriculography, which is used as anatomic localization mark to identify structure of positron emission tomography (PET) images of rat brain. Methods The rats were subjected to food deprivation prior to receiving tail vein injection of 18F-FDG. After an uptake period of 30 min, rats were placed in gantry and performed PET/CT scan in prone position. After the scan was completed, the rats were sacrificed under isoflurane anesthesia. A contrast agent was then perfused through the ascending aorta, and a head CT scan was performed again. The brain CT images after contrast agent injection were reconstructed. The PET and second scanned CT images were registrated and fused using PMOD software to observe the imaging of the ventricles and related structures, as well as the anatomical location of the activated brain regions in the PET images. Results The lateral ventricles, 3rd ventricle, aqueduct of midbrain and the 4th ventricle, the related structure to ventricles were clearly visualized in CT images. These structures could be used as landmark to locate the rat brain structure of PET images, such as A24a, A24a', caudate putamen, globus pallidus, accumbens nucleus, paratenial nucleus of thalamus. Conclusions Clear ventriculography plays a crucial role in the anatomical localization of PET images of the rat brain.
Key words
Ventriculography; /
PET; /
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CT; /
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Brain; /
/
Rat
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