Objective To investigate the effect of miR-19b-modified human umbilical cord mesenchymal stem cell (hUC-MSC) exosomes on immune balance in inflammatory bowel disease rats. Methods The hUC-MSC exosomes were identified. QRT-PCR was used to detect miR-19b expression in hUC-MSC exosomes. Rats were stochastically assigned into control group, inflammatory bowel disease group, hUC-MSC exosome group, mimic NC+hUC-MSC exosome group, and miR-19b mimic+hUC-MSC exosome group, each with 12. Except for the control group, inflammatory bowel disease models were established in all other groups, and after successful modeling, they were treated, once a day for 5 weeks. The colon length, colon pathology, spleen index, and thymus index were measured. Flow cytometry was used to test the proportions of peripheral blood helper T cells 17 (Th17), regulatory T cells (Treg), and Th17/Treg. ELISA was used to detect serum interleukin (IL)-10, transforming growth factor-β1 (TGF-β1), and IL-17. Results Compared with the hUC-MSC group and the mimic NC+hUC-MSC group, the miR-19b expression in exosomes was higher in the miR-19b mimic+hUC-MSC group (P<0.05). Compared with the control group, the inflammatory bowel disease group had colon edema, ulcers, disordered cell arrangement, abundant inflammatory cell infiltration, and shortened colon length, the spleen index, thymus index, proportion of Th17 and Th17/Treg in peripheral blood, serum IL-17 increased, while the proportion of Treg in peripheral blood, serum IL-10, and TGF-β1 decreased (P<0.05). Compared with the inflammatory bowel disease group, the hUC-MSC exosome group, mimic NC+hUC-MSC exosome group, and miR-19b mimic+hUC-MSC exosome group showed reduced colon edema, ulcers, disordered cell arrangement, and inflammatory cell infiltration in rats, and increased colon length, the spleen index, thymus index, proportion of Th17 and Th17/Treg in peripheral blood, serum IL-17 declined, while the proportion of Treg in peripheral blood, serum IL-10, and TGF-β1 increased (P<0.05). Compared with hUC-MSC exosome group and mimic NC+hUC-MSC exosome group, the miR-19b mimic+hUC-MSC exosome group showed improvement in colon edema, ulceration, disordered cell arrangement, and inflammatory cell infiltration, and an increase in colon length, the spleen index, thymus index, proportion of Th17 and Th17/Treg in peripheral blood, serum IL-17 declined, while the proportion of Treg in peripheral blood, serum IL-10, and TGF-β1 increased (P<0.05). Conclusions The miR-19b-modified hUC-MSC exosomes can promote Th17/Treg balance and improve immune function in inflammatory bowel disease rats.
Key words
miR-19b /
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Human umbilical cord-mesenchymal stem cells /
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Exosomes /
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Inflammatory bowel disease /
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T helper cell 17 /
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Regulatory T cells
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