Research and application on the development of a novel environmentally friendly alcoholic fixative for anatomy

doi:10.13418/j.issn.1001-165x.2025.6.17

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Chinese Journal of Clinical Anatomy ›› 2025, Vol. 43 ›› Issue (6) : 730-734. DOI: 10.13418/j.issn.1001-165x.2025.6.17

Research and application on the development of a novel environmentally friendly alcoholic fixative for anatomy

Zhao Bin¹, Zhang Tao¹, Li Shikun², Cheng Xiaoping², Li Xiaohe³*, Wang Zhiqiang³*, Wang Haiyan³

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Abstract

Objective To develop a novel environmentally friendly alcoholic fixative with superior efficacy, lower economic cost, and reduced irritancy, and to evaluate its preservation effectiveness. Methods A new fixative was formulated using a specific proportion of alcohol, glycerin, phenol, benzoic acid, ferric chloride, thymol, sodium chloride, and fragrance. This novel fixative was used to fix the cadavers of 12 four-month-old New Zealand white rabbits weighing 2000-2500 g for 120 days. Comparative analyses were conducted with traditional fixatives in terms of bacterial concentration, tissue specimen morphology, and economy. Additionally, a preservative irritancy experiment was performed on 20 female Wistar rats aged 7 to 8 weeks and weighing 170-180 g, comparing the irritancy of the novel fixative with that of traditional preservatives. Results Compared with traditional formalin fixatives, the novel environmentally friendly alcoholic fixative exhibited a longer fixation onset time but similar surface moldiness. It maintained good morphology of epithelial and muscle tissues, had equivalent bacteriostatic properties, and was less irritant and less expensive than most other improved fixatives. Conclusions The novel fixative has lower irritancy than traditional formalin preservatives and a lower price than most other improved fixatives, while exhibiting equivalent fixative capabilities to traditional formalin fixatives.

Key words

Formaldehyde-free fixative / / / Specimen fixation / / / Fixation effect / / / Ethanol

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Zhao Bin, Zhang Tao, Li Shikun, Cheng Xiaoping, Li Xiaohe, Wang Zhiqiang, Wang Haiyan. Research and application on the development of a novel environmentally friendly alcoholic fixative for anatomy[J]. Chinese Journal of Clinical Anatomy. 2025, 43(6): 730-734 https://doi.org/10.13418/j.issn.1001-165x.2025.6.17

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