Objective To prepare astragaloside IV hydrogel and explore the role of this dressing in the repair of full-thickness skin wounds in rats. Method Methacrylate anhydride modified gelatin hydrogels containing 0, 1.5 mg/mL, 2.0 mg/mL, and 2.5 mg/mL astragaloside IV were prepared, which were respectively denoted AS GelMA, GelMA/AS-IV-1.5, GelMA/AS-IV-2.0, and GelMA/AS-IV-2.5. Six full-thickness skin defects were made on the backs of 16 rats respectively. Astragaloside IV solution (2.5 mg/mL), GelMA hydrogel, GelMA/AS-IV-1.5 hydrogel, GelMA/AS-IV-2.0 hydrogel and GelMA/AS-IV-2.5 hydrogel were injected respectively, with a volume of 100 µL for each. One of the defect sites was not treated with injection (blank control group). The wound healing rate was observed on 3 d, 7 d, and 14 d after the operation. Hematoxylin-eosin staining was used to observe the re-epithelialization of the wound, Masson staining was used to observe the deposition of collagen in the wound, immunohistochemical staining was used to observe the angiogenesis of the wound, and immunofluorescence staining was used to observe scar hyperplasia, skin cell proliferation and the expression of Wnt/β-catenin signal transduction pathway proteins in the wound. The expressions of proliferating cell nuclear antigen and genes related to the Wnt/β-catenin signal transduction pathway were detected by RT-qPCR, and the expressions of proteins related to the Wnt/β-catenin signal transduction pathway were detected by Western blot. Results On 14 d after the operation, the wound healing rates of GelMA/AS-IV-1.5 hydrogel group and GelMA/AS-IV-2.0 hydrogel group were higher than those of blank control group (P<0.05). The thickness of granulation tissue in the wound and the effect of skin re-epithelialization in GelMA/AS-IV-2.0 hydrogel group were better than those in the other five groups. The expression level of CD31 in the wound was the highest, the expression of α -smooth muscle actinin was the lowest, and the protein and mRNA expression levels of cell proliferation nuclear antigen were the highest. The protein and mRNA expressions of β-catenin, LEF1 and c-Myc were the highest. Conclusion GelMA/AS-IV-2.0 hydrogel can accelerate wound healing by promoting skin cell proliferation, collagen deposition and angiogenesis, and inhibiting scar hyperplasia, and this process may be related to the activation of Wnt/β-catenin signaling pathway.
Key words
Astragaloside /
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Methacrylate gelatine /
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Hydrogel /
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Wound
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