Objective To analyze the intervention effect of gastrodia elata wall breaking powder in epileptic rats based on cyclic adenylate response element binding protein / brain-derived neurotrophic factor (CREB/BDNF) signaling pathway. Methods 38 SD male rats were divided into the control group (10), the model group (12), the drug control group (8) and Gastrodia elata wall powder group (8), respectively. The control and model groups were gavaged with saline. In the drug control group, 0.3 g/kg aqueous solution of piranpanet was given for gavage, and in the Gastrodia elata wall powder group, 1.0 g/kg powder solution was given. The behavior of rats was assessed. The duration of epilepsy was recorded. The rate of cell apoptotic was observed. The levels of TNF- α, IL-1 β, IL-6, CAT, MDA and SOD were measured. The memory ability of rats was assessed, and the levels of CREB / BDNF signaling pathway proteins were detected. Results Compared with the control group, the behavioral scores, cell apoptosis rate, TNF-α, IL-1 β, IL-6 and MDA level and the escape latency time in the model, drug control and Gastrodia elata wall powder groups were markedly increased (P<0.05). However, CAT and SOD levels, the number of platform crossing, p-CREB / CREB, and BDNF protein expression were all decreased (P<0.05). Compared with the model and the drug-control group, , the behavioral score, apoptosis rate, duration of attack, TNF- α, IL-1 β, IL-6, MDA level, and escape latency time in the Gastrodia elata wall powder group were all decreased (P<0.05), but CAT and SOD levels, times of platform crossing, p-CREB/CREB, and BDNF protein expression were all increased (P<0.05). Conclusions The wall-breaking powder of Gastrodia elata increases the activity of CREB/BDNF pathway and improves the memory ability of epileptic rats, inhibits the cell apoptosis rate, alleviates neuroinflammation and inhibits oxidative stress state.
Key words
Gastrodia elata wall-breaking powder /
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Cyclic adenylate response element binding protein / Brain-derived neurotrophic factor signaling pathway /
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Epilepsy /
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Rat
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