Specific regulation of Th9 differentiation and its potential immunological applications of Xanthiazone

doi:10.13418/j.issn.1001-165x.2025.5.11

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Chinese Journal of Clinical Anatomy ›› 2025, Vol. 43 ›› Issue (5) : 573-577. DOI: 10.13418/j.issn.1001-165x.2025.5.11

Specific regulation of Th9 differentiation and its potential immunological applications of Xanthiazone

Qiu Yuhui, Gao Yuan, Wang Handuo*

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Abstract

Objective To investigate the effects of xanthiazone on T cell differentiation. Methods Mouse T cells were sorted and cultured in vitro under conditions favoring differentiation into Th1, Th9, Th17, and Treg subsets. Xanthiazone was added to the cultures, and cytokine secretion and transcription factor expression were assessed by flow cytometry on the 3rd day. The pro-differentiation effects of xanthiazone on Th9 cells were further evaluated using a concentration gradient (10 nmol/L, 100 nmol/L, 200 nmol/L, 500 nmol/L, 1 μmol/L). Results Compared to the control group, xanthiazone selectively promoted Th9 differentiation without affecting Th1, Th17, and Treg differentiation. Furthermore, the concentration-dependent effects of xanthiazone on Th9 differentiation revealed that concentrations below 200 nM enhanced Th9 differentiation in a gradient-dependent manner. However, concentrations above 200 nM inhibited IL-9 secretion. Conclusions Higher concentrations of xanthiazone may suppress T cell activity, thereby antagonizing its pro-differentiation effects on Th9 cells.

Key words

Xanthiazone / / / Th9 / / / T cell differentiation

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Qiu Yuhui, Gao Yuan, Wang Handuo. Specific regulation of Th9 differentiation and its potential immunological applications of Xanthiazone[J]. Chinese Journal of Clinical Anatomy. 2025, 43(5): 573-577 https://doi.org/10.13418/j.issn.1001-165x.2025.5.11

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