The Protective Effect of Saposhnikovia divaricata Polysaccharide-Containing Serum on Neuronal Excitotoxicity and Neurotransmitter Release in PC12 Cells

doi:10.13418/j.issn.1001-165x.2025.5.08

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Chinese Journal of Clinical Anatomy ›› 2025, Vol. 43 ›› Issue (5) : 548-554. DOI: 10.13418/j.issn.1001-165x.2025.5.08

The Protective Effect of Saposhnikovia divaricata Polysaccharide-Containing Serum on Neuronal Excitotoxicity and Neurotransmitter Release in PC12 Cells

Zhang Dan¹, Yang Xiaoqian², Song Rui³, Sun Jinhao³, Xu Tao²*, Li Wenjie²*

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Abstract

Objective To investigate the neuroprotection of Saposhnikovia divaricata polysaccharides (SP) against glutamate (Glu)-induced excitotoxic injury and catecholamine (CA) neurotransmitters release in PC12 cells. Methods The cultured PC12 cells were treated with 15 mM Glu and the excitotoxicity injury model was established. SP-containing serum was prepared from rats administered with SP and added in culture plates. Cell viability was assessed via MTT assay. Intracellular ROS levels were detected with DCFH-DA probe. The effects of SP-containing serum on the single-vesicle CA storage, secretion, and exocytosis kinetics were detected using single-cell amperometry (SCA) and intracellular vesicle impact electrochemical cytometry (IVIEC). Vesicle ultrastructure was observed via transmission electron microscopy (TEM). Results SP-containing serum significantly improved the viability of Glu-damaged cells, reduced ROS content, increased CA storage and release at the single-vesicle level, and reversed the Glu - induced reduction in vesicle and dense core volumes. Conclusions SP exhibits obvious neuroprotective effect by alleviating oxidative stress, and SP can regulate CA release homeostasis by enhancing storage and release.

Key words

Saposhnikovia divaricata polysaccharides; / / Excitotoxicity; / / Oxidative stress; / / Electrochemistry; / / Catecholamine

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Zhang Dan, Yang Xiaoqian, Song Rui, Sun Jinhao, Xu Tao, Li Wenjie. The Protective Effect of Saposhnikovia divaricata Polysaccharide-Containing Serum on Neuronal Excitotoxicity and Neurotransmitter Release in PC12 Cells[J]. Chinese Journal of Clinical Anatomy. 2025, 43(5): 548-554 https://doi.org/10.13418/j.issn.1001-165x.2025.5.08

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