Establishment of an animal model for deep cervical lymphaticovenous anastomosis in rats

doi:10.13418/j.issn.1001-165x.2025.2.06

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Chinese Journal of Clinical Anatomy ›› 2025, Vol. 43 ›› Issue (2) : 147-150. DOI: 10.13418/j.issn.1001-165x.2025.2.06

Establishment of an animal model for deep cervical lymphaticovenous anastomosis in rats

Wu Jianlong¹, Xie Qingping²*, Chen Boda¹, Zhu Ziguan¹, Yang Xiaodong¹

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Abstract

Objective To establish a rat model of deep neck lymphatic vein anastomosis with complex anatomy and repeatable operation in response to the training needs of experimental animal microsurgery techniques, in order to enhance the operational ability of microsurgery physicians in lymphatic vein anastomosis in the deep neck anatomical area. Methods Twenty 4-5 week old SPF grade male SD rats (weighing approximately 100 g) were selected and anesthetized with pentobarbital sodium (45 mg/kg) via intraperitoneal injection, and then fixed in a supine position. Diluted indocyanine green (ICG) 0.05-0.1 mL (concentration: 0.1 mg/mL) was injected under the nasal mucosa to visualize lymphatic vessels, make a 1.5 cm incision along the midline of the neck, and intraoperative fluorescence imaging technology was used to expose deep cervical lymphatic vessels and external jugular veins. Lymphatic vein end-to-end anastomosis or end-to-end anastomosis at a magnification of 25 times was performed. Results The average diameter of the candidate lymphatic vessels in the deep neck was 0.18 mm (0.1-0.4 mm), and the diameter of the venous vessels was (0.6-1.2) mm. 20 cases of anastomosis were completed, with an immediate lymphatic vein recanalization rate of 95% (19/20) and 1 failure. Conclusions This model can simulate the clinical scenario of deep neck lymphatic vein anastomosis, and has both anatomical preparation and operational repeatability, providing an operable animal model for training deep neck lymphatic vein anastomosis.

Key words

Deep cervical lymphaticovenous anastomosis / / / Animal model / / / Microsurgery / / / Skill training

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Wu Jianlong, Xie Qingping, Chen Boda, Zhu Ziguan, Yang Xiaodong. Establishment of an animal model for deep cervical lymphaticovenous anastomosis in rats[J]. Chinese Journal of Clinical Anatomy. 2025, 43(2): 147-150 https://doi.org/10.13418/j.issn.1001-165x.2025.2.06

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