Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells

doi:10.13418/j.issn.1001-165x.2023.5.08

PDF(9258 KB)

Chinese Journal of Clinical Anatomy ›› 2023, Vol. 41 ›› Issue (5) : 543-549. DOI: 10.13418/j.issn.1001-165x.2023.5.08

Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells

Zheng Kai ¹, Luo Xiuling², Zhang Weihao¹, Liu Yuli ¹, Li Yuming ¹, Liao Shanggao³

Author information +

History +

Abstract

Objective To explore the effect and potential mechanism of lncRNA NEAT1 on the invasion and migration of pituitary tumor cells. Methods The expressions of lncRNA NEAT1, miR-134, and ITGB1 were detected by qRT-PCR. Transwell was used to detect the invasion and migration. The regulatory relationship between miR-134 and lncRNA NEAT1 and ITGB1 was detected by a double luciferase reporter gene experiment. The expression of ITGB1/FAK/PI3K pathway-related proteins was detected by Western blot. Results Knockdown of lncRNA NEAT1 inhibited the invasion and migration of GH3 cells (P<0.05); inhibition of miR-134 could reverse the inhibitory effect of down-regulated lncRNA NEAT1 on the invasion and migration of GH3 cells (P<0.05). miR-134 had a negative targeted regulation relationship with lncRNAs NEAT1 and ITGB1 (P<0.05). Up-regulation of miR-134 inhibited the invasion and migration of GH3 cells and inhibited the activation of the ITGB1/FAK/PI3K pathway (P<0.05). ITGB1 overexpression attenuated the effect of up-regulated miR-134 on GH3 cells (P<0.05). Conclusions Down-regulation of lncRNA NEAT1 targeting miR-134 may inhibit the invasion and migration of pituitary tumor cells by inhibiting the activation of the ITGB1/FAK/PI3K pathway.

Key words

lncRNA NEAT1 / 　miR-134 / 　Pituitary tumor / 　Invasion / 　Migration

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Zheng Kai, Luo Xiuling, Zhang Weihao, Liu Yuli, Li Yuming, Liao Shanggao. Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells[J]. Chinese Journal of Clinical Anatomy. 2023, 41(5): 543-549 https://doi.org/10.13418/j.issn.1001-165x.2023.5.08

References

[1] Wang L, Cho KB, Li Y, et al. Long Noncoding RNA (lncRNA)-mediated competing endogenous RNA networks provide novel potential biomarkers and therapeutic targets for colorectal cancer[J]. Int J Mol Sci, 2019, 20(22): 5758-5764. DOI: 10.3390/ijms20225758.
[2] Li X, Deng S, Pang X, et al. lncRNA NEAT1 silenced miR-133b promotes migration and invasion of breast cancer cells[J]. Int J Mol Sci, 2019, 20(15): 3616-3621. DOI: 10.3390/ijms20153616.
[3] 庞其军, 赵颖, 郗艳国, 等. miR-134在人垂体腺瘤组织中的表达及其意义[J]. 现代生物医学进展, 2014, 14(17): 3328-3333. DOI: 10.13241/j.cnki.pmb.2014.17.034.
[4] 马丽媛, 代从新. 抗血管内皮生长因子靶向治疗难治性垂体腺瘤和垂体腺癌研究进展[J]. 中国神经精神疾病杂志, 2020, 46(10): 625-628. DOI: 10.3969/j.issn.1002-0152.2020.10.011.
[5] Beylerli O, Khasanov D, Gareev I, et al. Differential non-coding RNAs expression profiles of invasive and non-invasive pituitary adenomas[J]. Noncoding RNA Res, 2021, 6(3): 115-122. DOI: 10.1016/j.ncrna.2021.06.004.
[6] Wang X, Li X, Wang Z. lncRNA MEG3 inhibits pituitary tumor development by participating in cell proliferation, apoptosis and EMT processes[J]. Oncol Rep,2021,45(4): 40-53.DOI: 10.3892/or.2021. 7991.
[7] 倪文. lncRNA MIR4435-2HG靶向miR-1-3p调控信号通路PI3K/Akt对胰腺癌细胞增殖,侵袭和迁移的机制研究[J]. 安徽医药, 2021, 25(3): 467-473. DOI: 10.3969/j.issn.1009-6469.2021.03.009.
[8] Kou JT, Ma J, Zhu JQ, et al. lncRNA NEAT1 regulates proliferation, apoptosis and invasion of liver cancer[J]. Eur Rev Med Pharmacol Sci, 2020, 24(8): 4152-4160. DOI: 10.26355/eurrev_202004_20995.
[9] 周建华, 胡涛, 陈宇, 等. lncRNA NEAT1在人脑胶质瘤的表达变化及其与病人预后的关系[J]. 中国临床神经外科杂志, 2019, 24(3): 148-150. DOI: 10.13798/j.issn.1009-153X.2019.03.007.
[10]Wang W, Ge L, Xu XJ, et al. lncRNA NEAT1 promotes endometrial cancer cell proliferation, migration and invasion by regulating the miR-144-3p/EZH2 axis[J]. Radiol Oncol, 2019, 53(4): 434-442. DOI: 10.2478/raon-2019-0051.
[11]Chen CL, Zhang L, Jiao YR, et al. miR-134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP1 and MMP3 in vitro and in vivo[J]. FEBS Lett, 2019, 593(10): 1089-1101. DOI: 10.1002/1873-3468.13387.
[12]Ellert-miklaszewska A, Poleszak K, Pasierbinska M, et al. Integrin signaling in glioma pathogenesis: from biology to therapy[J]. Int J Mol Sci, 2020, 21(3): 888-893. DOI: 10.3390/ijms21030888.
[13]Wang N, Chang LL. Maspin suppresses cell invasion and migration in gastric cancer through inhibiting EMT and angiogenesis via ITGB1/FAK pathway[J]. Hum Cell, 2020, 33(3): 663-675. DOI: 10.1007/s13577-020-00345-7.
[14]Wu YJ, Lin SH, Din ZH, et al. Sinulariolide inhibits gastric cancer cell migration and invasion through downregulation of the EMT process and suppression of FAK/PI3K/AKT/mTOR and MAPKs signaling pathways[J]. Mar Drugs, 2019, 17(12): 668-874. DOI: 10.3390/md17120668.