Displaying the nerve distribution of SD rat skin by a further modified Sihler's intramuscular nerve staining method
SHI Quan, WEI Zai-rong, YANG Sheng-bo, JIN Wen-hu, LI Hai, WANG Da-li, WANG Bo
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (4) : 461-464.
Displaying the nerve distribution of SD rat skin by a further modified Sihler's intramuscular nerve staining method
Objective To investigate the application of the further modified Sihler's intramuscular nerve staining method in displaying the nerve distribution of SD rat skin. Methods After six adult SD rats were selected and sacrificed, the skin was harvested against muscle surface on the dorsal and ventral side. Finally, 6 pieces of the dorsal skin and 6 pieces of the ventral skin were obtained. After fixation in 10% formaldehyde solution for 1 month, the skins were defatted by anhydrous ethanol for 3 days, and digested in 0.25% collagenaseⅠand Ⅲ solution for 3 days. After these steps, the skins were stained using the further modified Sihler's intramuscular nerve staining method. Results A total of 12 pieces of SD rat skin were stained, among which 10 pieces obtained a good staining result. In the process of removing the pigment, the epidermis often fell off and even if it did not fall off, a boundary between the epidermis and dermis could not be found on the tiled skin and the nerve branches in the epidermis could not be seen; it was difficult to remove partial subcutaneous fat completely in the anatomical process. Therefore, obvious nerve distribution in partial subcutaneous fat was visible to naked eyes. By virtue of X-Ray film viewer, it could be seen by naked eyes that the skin was light purple in color, and the nerves were black in color and had an uneven distribution with different shape and diameter. It could even be seen that the nerves were twisted and knotted. Conclusion Skin nerve distribution visible to naked eyes can be clearly displayed after SD rat skin is stained by the further modified Sihler 's intramuscular nerve staining method. These results have a certain significance for the determination of nerve fiber density in each region of SD rat skin and the study of nerve regeneration after skin flap transplantation in animal models.
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