Preparation and identification of liver specific DEK knockout mice
WANG Chao-xuan1, HOU Yu2, CHEN Zhe2, SUN Hang1
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (4) : 397-401.
Preparation and identification of liver specific DEK knockout mice
Objective To establish a stable liver-specific DEK knockout mice and provide the important animal model for further studying the biological function of DEK gene in the liver. Methods Mating and identification of DEKflox/flox mice with Alb-Cre mice were carried out, and the DEKflox/+/Alb-Cre mice were screened. Mating and identification of DEKflox/+/Alb-Cre mice with DEKflox/flox mice was carried out, and the DEKflox/flox/Alb-Cre mice were screened. Mating and identification of DEKflox/flox/Alb-Cre mice with DEKflox/flox mice were carried out, and screening the DEKflox/flox/Alb-Cre mice was performed. The DEKflox/flox/Alb-Cre mouse was a liver-specific DEK knockout mouse and the DEKflox/flox mouse was a control mouse. The mouse genotypes were identified by PCR. We used Real-Time PCR and Western Blotting to detect the expression of DEK mRNA and protein levels in mouse liver. We used Immunofluorescence to detect DEK gene expression in mouse liver. The hepatic tissue morphology of the mouse liver was observed using hematoxylin-eosin staining. Results PCR results indicate that mouse genotypes are consistent with DEKflox/flox/Alb-Cre. Compared with DEKflox/flox mice, DEK mRNA and protein levels were significantly reduced, and liver tissue morphology showed no significant difference. Conclusion In this study, the liver-specific DEK knockout mice are successfully constructed using the Cre/Loxp technology, providing an important animal model for further study of the role of DEK genes.
DEK / Cre/Loxp / Liver / Gene knockout
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