Changes of dopamine receptor 1 and dopamine receptor 2 expression in dorsal motor nucleus of vagus induced by substantia nigra lesion by lipopolysaccharide
LIAN Hui, ZHANG Yi-min, FU Sheng-qi, WANG Zhi-yong
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3) : 286-290.
Changes of dopamine receptor 1 and dopamine receptor 2 expression in dorsal motor nucleus of vagus induced by substantia nigra lesion by lipopolysaccharide
Objective To observe the expression of tyrosine hydroxylase (TH)、dopamine receptor 1 (DR1) and dopamine receptor 2 (DR2) in rat dorsal motor nucleus of vagus (DMV) induced by substantia nigra (SN) lesion by lipopolysaccharide (LPS). Methods According to the random number table, a total of 30 female SD rats were randomly divided into a LPS group and a normal control group. LPS was injected into the bilateral SN of the rats in the LPS group, and the same volume of saline was injected into the same position of the rats in the normal control group. After six weeks, rotating rod experiment, gastric emptying, immunofluorescent assay and western blotting analysis were used to detect TH expression in the SN and TH, DR1 and DR2 expression in the DMV. Results Six weeks later，the LPS group rats were identified successfully, and the gastric emptying ability was reduced, the number of TH immunoreactive neurons in the DMV of the LPS group increased from (7±3) to (12±2) (P<0.05), the number of DR1 immunoreactive neurons decreased from (28±5) to (18±6) (P<0.05) and the number of DR2 immunoreactive neurons increased from (20±3) to (33±4) (P<0.05). In the dorsal medulla, the level of TH protein increased from (0.16±0.02) to (0.43±0.04) (P<0.05), the level of DR1 protein decreased from (0.78±0.05) to (0.36±0.03) (P<0.05) and the level of DR2 protein increased from (0.43±0.03) to (0.58±0.07) (P<0.05). Conclusion The changes of TH, DR1 and DR2 expression in DMV of LPS group rats may be associated with gastric emptying dysfunction in patients with Parkinson’s disease.
LPS； Dorsal motor nucleus of vagus； Dopamine receptor 1; / Dopamine receptor 2
[1] Pellegrini C, Antonioli L, Colucci R, et al. Gastric motor dysfunctions in Parkinson’s disease: Current pre-clinical evidence[J]. Parkinsonism Relat Disord, 2015, 21(12):1407-1414.
[2] Cai QQ,Zheng LF,Fan RF,et al. Distribution of dopamine receptors D1- and D2-immunoreactive neurons in the dorsal motor nucleus of vagus in rats[J]. Auton Neurosci, 2013, 176(1-2):48-53.
[3] Zheng Z, Travagli RA. Dopamine effects on identified rat vagal motoneurons[J]. Am J Physiol Gastrointest Liver Physiol, 2007, 292(4):1002-1008.
[4] Lehnardt S, Massillon L, Follett P, et al. Activation of innate immunity in the CNS triggers neurodegeneration through a Toll - like receptor 4-dependent pathway[J].Proc Natl Acad Sci USA, 2003,100(14):8514 -8519.
[5] Walters JR, Hu D, Itoga CA, et al. Phase relationships support a role for coordinated activity in the indirect pathway in organizing slow oscillations in basal ganglia output after loss of dopamine[J]. Neuroscience, 2007, 144(2):762-776.
[6] Babic T, Browning KN, Travagli RA. Differential organization of excitatory and inhibitory synapses within the rat dorsal vagal complex[J].Am J Physiol Gastrointest Liver Physiol, 2011, 300(1):21-32.
[7] Baldessarini RJ, Tarazi FI. Brain dopamine receptors:A primer on their current status, basic and clinical[J]. Harv Rev Psychiatry,1996, 3(6):301-325.
[8] Matsuda Y, Marzo A, Otani S. The presence of background dopamine signal converts long-term synaptic depression to potentiation in rat prefrontal cortex[J].J Neurosci, 2006, 26(18):4803-4810.
[9] Floresco SB, Magyar O. Mesocortical dopamine modulation of executive functions: Beyond working memory[J]. Psychopharmacology(Berl), 2006, 188(4):567-585.
[10] Shuai XW, Xie PY. Expression and localization of c-Fos and NOS in the central nerve system following esophageal acid stimulation in rats[J].World J Gastroenterol, 2004,10(15):2287-2291.
[11]Browning KN. Role of central vagal 5-HT3 receptors in gastrointestinal physiology and pathophysiology[J]. Front Neurosci, 2015, 29(9):413.
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