Synthesis and characterization of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres and observation of its inhibition on MG63 cells
YANG Lian-Jun, RONG Zi-Jie, ZHANG Zan-Jie, WU Guo-Feng, ZHANG Xi-Bing, WANG Jian, SHU Li-Xin
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (5) : 585-589.
Synthesis and characterization of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres and observation of its inhibition on MG63 cells
Objective To study preparation and characteristics of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres(ADM-PLGA-NHAC) and observe its in-vitro anti-tumor effect. Method ADM-PLGA-NHAC were prepared by freezing and lyophylization of composite solution.The characteristic and morphology of the scaffolds were observed by scanning electron microscope, and the release behavior of ADM was investigated in vitro. The antitumor activities of ADM-PLGA-NHAC against human MG63 cell lines were detected by CCK8 colorimetric assay and live/dead cell staining. Result The ADM-PLGA microspheres couldbe uniformly distributed in nano-hydroxyapatite/collagen scaffolds to form ADM-PLGA-NHAC scaffolds, which had 100-200 μm pore diameter and over 82% porosity. The release study indicated that the ADM-PLGA-NHAC show sustained release behavior in vitro. The leaching liquor of ADM-PLGA-NHAC could inhibit MG63 cell lines proliferation in a dose-dependent manner. Conclusion The ADM-PLGA-NHAC shows sustained release characteristics and anti-tumor effect, and it will have a wide application prospect in the fields of osteosarcoma.
Hydroxyapatite / Collagen / Adriamycin / Nanoparticles / Osteosarcoma / Porous scaffold
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