收稿日期: 2016-09-02
网络出版日期: 2017-04-27
基金资助
重庆市研究生科研创新项目（40010200100410）; 国家青年科学基金（81403466）
Expression changes of pEGFR after decompression of compressed spinal cord injury in rats
Received date: 2016-09-02
Online published: 2017-04-27
目的 探讨大鼠脊髓压迫性损伤（compressed spinal cord injury，CSCI）解压后表皮生长因子受体（phosphorylated epidermal growth factor receptor，pEGFR）、pAkt1的表达变化及其与神经功能、有髓神经纤维数量变化的相关性，为CSCI解压后治疗策略的制定和药物的研发提供实验基础。 方法 采用自行设计的方法制作SD大鼠CSCI模型，造模成功后解压。运用BBB（Basso Beattie Bresnahan）评分观察动物解压后神经功能的恢复情况；通过Luxol fast blue（LFB）染色检测解压后1、7、14、21 d有髓神经纤维数量变化；运用免疫荧光双标（Double-labeling immunoflurescence）、免疫印迹（western blotting，WB）检测pEGFR、 pAkt1的表达变化。 结果 CSCI解压后，BBB评分和有髓神经纤维数量随时间延长而逐渐增加；与此同时，pEGFR、pAkt1表达亦上调且与BBB评分、有髓神经纤维数量增加趋势一致。 结论 CSCI解压后，神经功能有一定改善、有髓神经纤维数量有一定增加，这些变化与pEGFR表达上调有关，提示EGFR的活化参与了CSCI解压后的内源性修复。
龚睿 , 孙善全 , 钟源 , 张威 , 赵琪 , 牟科杰 , 薛军 . 大鼠脊髓压迫性损伤解压后pEGFR的表达变化[J]. 中国临床解剖学杂志, 2017 , 35(2) : 166 -171 . DOI: 10.13418/j.issn.1001-165x.2017.02.010
Objective To investigate the changes of pEGFR, pAkt1 expression in rat after decompression of compressed spinal cord injury(CSCI), and its correlation with nerve structure and function change，providing experimental basis for the development of therapeutic strategies and the development of medication for the treatment of CSCI after decompression. Methods The CSCI model was established first with a self-made device, which then underwent spinal decompression. The motor functions were monitored by Basso, Beattie & Bresnahanlocomotor rating scale. The pathological changes in axonal myelinated fibers were estimated by luxol fast blue (LFB). Epidermal growth factor receptor (EGFR), and phosphorylated Akt1 (pAkt1) were detected by double-labeling immunofluorescence and western blotting assays. Results The motor functions and number of myelinated nerve fibers were increased along with time extending after decompression. The expression of EGFR and pAkt1 was also increased after decompression, which was consistent with the changes of motor functions and number of myelinated nerve fibers. Conclusion The structure and function of the injured nerve can be restored to a certain extent, and the expression of pEGFR is closely related to this phenomenon after decompression. It suggests that the activation of EGFR is involved in the endogenous repair of CSCI after decompression.
Key words： Compressed spinal cord injury; Neural myelin sheaths; Demyelination; EGFR; pAkt1
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