单羧酸转运蛋白1、2、4在急性心衰大鼠模型肾上腺的表达变化
收稿日期: 2014-06-18
网络出版日期: 2015-04-21
基金资助
重庆医科大学本科生创新实验资助项目 （201202）； 国家自然科学基金（8100056）
Alterations of MCT 1、2、4 expression in adrenal gland of rat with acute heart failure
Received date: 2014-06-18
Online published: 2015-04-21
目的 观察单羧酸转运蛋白（monocarboxylate transporter , MCT）1、2、4在急性心衰大鼠肾上腺的表达变化，以探讨急性心衰肾上腺的能量代谢变化。 方法 随机将成年健康SD大鼠（雌雄不限， 250±20 g）50只，分为3组： 正常组（n=10）、假手术组（n=20，2 subgroups, 10 in each）和模型组（n=20，2 subgroups, 10 in each）。用1.5%戊巴比妥钠静脉注射制作大鼠急性心衰模型；注毕，运用BL-420F生物机能实验系统记录左室内压峰值（LVSP）和左室内压最大上升速率（+dp/dtmax）的变化曲线；用IHC和Western Blot检测模型鼠，肾上腺MCT1，2，4的表达变化。 结果 在急性心衰发生10 min时，肾上腺皮质和髓质MCT1、2、4的表达均有升高，此时假手术组MCT1、2、4也有升高。与正常对照组相比较，两组的MCT1、2、4的表达升高均具有统计学意义（P<0.05）；在20 min时，大鼠肾上腺MCT1、2、4的表达较正常组却有所下降，与正常组相比较，仅MCT1的表达下调具有统计学意义（P<0.05），而MCT2、4 则无明显差别（P>0.05）。假手术20 min组与10 min组相比较，MCT1、2、4的表达无明显差别（P<0.05）。 结论 在急性心衰和创伤性应激状态下，肾上腺MCT1、2、4的表达，在十分钟内呈代偿性增加，但随着心衰时间的延长，其表达反而降低，机体的能量代谢出现失代偿，提示MCT1、2、4参与创伤性应激及急性心衰的能量代谢过程。
关键词： 单羧酸转运蛋白1、2、4; 急性心衰; 应激; 肾上腺
何书娟 , 孙善全 , 马容 , 王静文 , 蒋徐丽 , 宋开琴 . 单羧酸转运蛋白1、2、4在急性心衰大鼠模型肾上腺的表达变化[J]. 中国临床解剖学杂志, 2015 , 33(2) : 193 -198 . DOI: 10.13418/j.issn.1001-165x.2015
Objective To observe the alterations of the MCT 1、2、4 expression in adrenal gland of rats with acute heart failure , so as to explore the alterations of energy metabolism of adrenal gland under stress of acute heart failure. Method Fifty healthy adult SD rats were randomly divided into 3 groups: normal, sham(2 subgroups) and experimental groups(2 subgroups). In experimental group, acute heart failure model was established by 1.5% sodium pentobarbital injection i.v . In sham group, the rats underwent the same surgical procedures as those in experimental group with NS instead of 1.5% sodium pentobarbital injection. After injection, left ventricular systolic pressure（LVSP）and increased rate of left ventricular pressure maximum（+dp/dtmax）were recorded to judge whether the model was successful or not. The animals were sacrificed and their adrenal glands were properly taken at 10 and 20 min after the models had been established successfully. Immunohistochemistry（IHC） and Western Bolt(WB) methods were used to localize and detect the alterations of MCT1、2、4 expression. Results IHC and WB results showed that the expressions of MCT1、2、4 were statistically increased in both experimental and sham groups in 10 min(P<0.05), compared with the normal group. The semi-quantitative analysis of MCT1、2、4 showed that in 20min the amount of MCT1、2、4 expression in experimental group was lower than that in control group (MCT1:P<0.05; MCT2 & MCT4: P>0.05). Conclusions Under the conditions of acute heart failure and traumatic stress, the upregulation of MCT1、2、4 expression in adrenal gland might make a compensation for energy consumption within 10 min. However, the capacity of compensation was limited. The results suggest that MCT1、2、4 might play an important role in stress reaction .
Key words： MCT1、2、4; Acute Heart Failure; Stress; Adrenal gland
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