脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究
收稿日期: 2014-05-05
网络出版日期: 2015-04-21
基金资助
泸州医学院青年基金项目（2011037）；泸州市科技局基金项目（泸市科【2013】134号）
The experimental study on proliferation and migration pathway of the neural stem cells in the rats following intracerebral hemorrhage
Received date: 2014-05-05
Online published: 2015-04-21
汤华军 , 范光碧 , 郑宇杰 , 邓莉 , 高小青 , 杨朝鲜 . 脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究[J]. 中国临床解剖学杂志, 2015 , 33(2) : 189 -192 . DOI: 10.13418/j.issn.1001-165x.2015
Objective To investigate proliferation and migration pathways of the neural stem cells in the rats following intracerebral hemorrhage (ICH). Methods The 75 SD rats were randomly divided into the ICH group, sham group and normal group the ICH group received injection of autologous blood to establish the ICH model. The bromodeoxyuridine (BrdU) was used to mark neural stem cells. Proliferation, migration and distribution of neural stem cells were observed by immunohistochemical staining. Results BrdU-positive cells located in the ependymal epithelium of subventricular zone increased significantly in the ICH group compared with the control group and normal group( P<0.05), and migrated along corpus callosum, and finally settled in the hemorrhagic focus and the surrounding area. Conclusion Neural stem cells in the rats following ICH can proliferate and migrate to the hemorrhagic focus and surrounding area along corpus callosum, and participate in repairing the nerve function of hemorrhagic brain injury.
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