目的　建立帕金森病(Parkinson’s disease, PD)大鼠模型，观测和评价其行为学改变， 检测和分析PD大鼠模型黑质-纹状体通路中单胺类神经递质含量变化。  方法　采用6-羟基多巴胺立体定向注射至成年SD大鼠单侧前脑内侧束，制作偏侧PD大鼠模型，观测和评价其行为学改变(悬尾测试、旋转行为的旋转速度、启动时间和维持时间)，通过高效液相色谱法测定和分析单胺类神经递质的含量在PD大鼠模型黑质-纹状体通路中的变化。  结果　(1) PD大鼠出现了尾僵直、少动、运动迟缓、震颤、竖毛、咬尾或足、姿势和步态不稳等异常行为；(2) 悬尾测试对于评价PD模型没有统计学意义，旋转行为的旋转速度、启动时间和维持时间的结果具有统计学意义且呈时间依赖性；(3) PD大鼠模型毁损侧的黑质与纹状体部位的多巴胺(Dopamine, DA)和五羟色胺(Serotonin, 5-HT)含量均降低，DA的含量具有逐渐下降的趋势，术后第2、3、4及5周黑质部位的DA含量相对于正常对照组分别下降62.96%、82.88%、92.71%、91.50%，纹状体部位的DA含量分别下降77.47%、 91.39%、96.25%、96.18%；术后第3、4及5周黑质部位的DA含量平均下降(89.03±5.36)% (第3、4及5周的DA含量无统计学差异)，纹状体部位的DA含量平均下降(93.61±2.79)% (第3、4及5周的DA含量无统计学差异)。  结论　(1) PD大鼠模型具有类似人类PD的运动症状，除旋转速度作为旋转行为经典的行为学指标外，启动时间和维持时间可作为PD模型旋转行为的辅助指标；(2) PD大鼠脑内毁损侧的黑质和纹状体部位的DA和5-HT含量均降低。

Objective  To study the changes in levels of monoamine neurotransmitter within the nigrostriatal pathway and evaluate the behavioral changes in rat models of Parkinson's disease.  Method  The unilateral rat models of PD were established by injecting 6-OHDA into the right medial forebrain bundle of adult Sprague-Dawley rats. After establishing the successful rat models, we observed their abnormal behavior and evaluated the behavioral changes (elevated body swing test, average velocity, actuation time and time length of rotation behavior). Additionally, we assayed the levels of monoamine neurotransmitter in the nigrostriatal pathway of PD rats by high performance liquid chromatography.  Results  (1) PD rats demonstrated some abnormal behavior such as stiffness of tail, akinesia or bradykinesia, tremor and the instable posture and gait, etc. (2) Statistically, elevated body swing test was not significant for the evaluation of PD rat models, while average velocity, actuation time and time length of rotation behavior were significant for the evaluation and showed a time-dependent pattern. (3) Both of the content of DA and serotonin were reduced in the substantia nigra and the striatum of the pathological lateral in PD rats, and the DA content decreased with time; Moreover, the degree of decline in DA content of the substantia nigra were respectively 62.96%, 82.88%, 92.71% and 91.50% and the striatum were respectively 77.47%, 91.39%, 96.25% and 96.18% during the second week, third week, fourth week and the fifth week. The average degree of decline in DA content of the substantia nigra was (89.03±5.36)% during the third week, fourth week and the fifth week by statistical analysis, while the striatum was (93.61±2.79)%.  Conclusions  (1) PD rats have the human-like clinical motor symptoms of PD, and the classic behavioral evaluation index of rotational behavior is average velocity, while actuation time and time length of rotation behavior play a supporting role in behavior evaluation of PD rat models; (2) There is a decline in the content of DA and serotonin of the substantia nigra and the striatum in the lesioned lateral of PD rats.