猪到食蟹猴异位小肠移植模型的建立
收稿日期: 2014-02-26
网络出版日期: 2015-02-12
基金资助
国家自然科学基金（81270945）
Establishment of heterotopic intestinal xenotransplantation model from pig to cynomolgus
Received date: 2014-02-26
Online published: 2015-02-12
目的 探讨稳定的猪到食蟹猴异位小肠移植模型建立方法，为异种小肠移植排斥反应的研究提供良好的实验工具。 方法 分别以白色杂种猪和食蟹猴做供、受体，采用供体小肠的肠系膜前动、静脉分别与受体肾下腹主动脉和肾下下腔静脉行端侧吻合，供体远、近端肠管结扎的方式建立异种节段性小肠移植模型。 结果 共行猪到食蟹猴异种节段小肠移植5例，移植肠肠长度为(52.0±5.7) cm，血管吻合成功率为100%，移植肠存活时间为(152±72) min(55~245 min)。结论 本研究建立了猪到食蟹猴的异位小肠移植模型，模型稳定，可复性强，为进一步研究转基因猪到食蟹猴小肠移植提供了理想的平台。
姚丹华 , 李幼生 , 王剑 , 毛琦 , 郭明晓 , 张少一 , 孔文成 , 任乐乐 , 黎介寿 . 猪到食蟹猴异位小肠移植模型的建立[J]. 中国临床解剖学杂志, 2015 , 33(1) : 81 -84 . DOI: 10.13418/j.issn.1001-165x.2015
Objective To investigate the procedures for establishing a stable model of intestinal xenotransplantation from pig to cynomolgus, with the purpose of providing a good experimental tool for the xenograft rejection study in intestinal xenotransplantation. Methods White wild-type pigs were used as donor and cynomolguses used as recipients. The procedure of segmental heterotopic intestinal xenotransplantation in recipients was performed as following: the donor`s anterior mesenteric artery was anastomosed end-to-side to the recipient`s infrarenal abdominal aorta, and the donor`s tributary of anterior mesenteric venous was anastomosed end-to-side to the recipient`s infrarenal inferior vena cave. Subsequently, both the proximal and distant ends of the graft intestine were stayed ligated. Results Segmental intestinal xenotransplantation was performed in 5 cynomolguses, with an average graft intestine length of (52.0±5.7) cm. The success rate of vascular anastomosis was 100%, and the intestinal xenograft survival was 152±72(55~245) minutes. Conclusions A stable, repeatable model of segmental intestinal xenotransplantation from pig to cynomolgus was established, which would provide an ideal tool for further research on intestinal xenotransplantation from transgenic pig to cynomolgus.
Key words： Lumbar spondylolysis; Slipping prophase; Lumbar instability
[1] Starzl TE, Marchioro TL, Peters GN, et al. Renal Heterotransplantation from Baboon to Man: Experience with 6 Cases
[J].Transplantation, 1964, 2: 752-776.
[2] Hardy JD, Chavez CM. The first heart transplant in man: historical reexamination of the 1964 case in the light of current clinical experience
[J]. Transplant Proc, 1969, 1(2): 717-725.
[3] Starzl TE, Fung J, Tzakis A, et al. Baboon-to-human liver transplantation
[J]. Lancet, 1993, 341(8837): 65-71.
[4] Dai Y, Vaught TD, Boone J, et al. Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs
[J]. Nat Biotechnol, 2002, 20(3): 251-255.
[5] Zeyland J, Wo?niak A, Gawrońska B, et al. Double transgenic pigs with combined expression of human α1,2-fucosyltransferase and α-galactosidase designed to avoid hyperacute xenograft rejection
[J]. Arch Immunol Ther Exp (Warsz), 2014, 62(5):411-422.
[6] Pierson RN, 3rd, Dorling A, Ayares D, et al. Current status of xenotransplantation and prospects for clinical application
[J]. Xenotransplantation, 2009, 16(5): 263-280.
[7] Yedidag EN, Fryer JP, Levi E, et al. Early histopathology of small intestinal discordant xenografts
[J]. Transplantation, 1996, 62(10): 1385-1391.
[8] Kiyochi H, Kellersmann R, Blomer A, et al. Rat-to-mouse small bowel xenotransplantation: a novel model for studying acute vascular and hyperacute xenograft rejection and xenogenic cell migration
[J]. Xenotransplantation, 1999, 6(1): 28-35.
[9] Yandza T, Tauc M, Saint-Paul MC, et al. The pig as a preclinical model for intestinal ischemia-reperfusion and transplantation studies
[J]. J Surg Res, 2012, 178(2): 807-819.
/
| 〈 |
|
〉 |
京ICP备08002354号-3
