短期双眼视觉剥夺对大鼠视网膜影响的蛋白组学研究
收稿日期: 2014-03-24
网络出版日期: 2015-02-12
Proteomic study on influence of short-term binocular visual deprivation on the rat retina
Received date: 2014-03-24
Online published: 2015-02-12
目的 探讨短期视觉剥夺对视网膜蛋白表达的影响。 方法 在大鼠出生后14 d，行双眼睑缝合14d，建立视觉剥夺模型。通过二维凝胶电泳分离视网膜蛋白，用PDQuest 软件选择差异表达的蛋白，用质谱仪鉴定表达差异的蛋白，并用Western blot和免疫组织化学法进一步确定差异蛋白的变化，用HE染色观察视网膜细胞的变化。结果 视觉剥夺后，视网膜中4个蛋白的表达量下降超过（2.5±0.5）倍。它们分别是热休克蛋白70、糖酵解蛋白α-烯醇化酶、转运蛋白血清铁传递蛋白和钙离子结合蛋白恢复蛋白。然而未发现视网膜细胞数量的减少。结论 短期视觉剥夺可以引起视网膜中蛋白的变化，可能与弱视的产生有密切关系。
何文姬 , 柳佳利 , 郑发武 , 宿宝贵 , 潘三强 . 短期双眼视觉剥夺对大鼠视网膜影响的蛋白组学研究[J]. 中国临床解剖学杂志, 2015 , 33(1) : 76 -80 . DOI: 10.13418/j.issn.1001-165X.2015
Objective To explore the influence of visual deprivation on retinal protein expression. Methods The visual deprivation model is established by bilateral eyelid closure. Bilateral eyelid closure was performed at the postnatal 14 days, 2 weeks after which the rats were used for further experiment Two-dimensional gel electrophoresis was used to separate proteins in the retina, the differential proteins were chosen using PDQuest software and identified by tandem MS. the change of protein expression was further confirmed by Western blot and immunohistochemistry. Hematoxylin and eosin staining was performed to observe the retinal structure. Results Expressions of four proteins in the rat retina were decreased after visual deprivation. These proteins were identified as heat shock 70、alpha-enolase、serotranfferin and recoverin. However, we did not observe the change of the amoun of the retinal cells under the light microscope. Conclusions Short-term visual deprivation induces the change of retinal proteins, and may contribute to the occurrence of amblyopia.
Key words： Visual deprivation; Retina; Proteomics
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