不同空间排列静电纺丝对RSC96细胞表达NGF及Laminin的研究
收稿日期: 2014-04-27
网络出版日期: 2015-02-12
基金资助
澳门科学技术发展基金（016-2009-A1）
The influence of topography of electrospun fibrous scaffolds on NGF and Laminin expressions of RSC96 cells
Received date: 2014-04-27
Online published: 2015-02-12
目的：研究不同空间排列静电纺丝对雪旺细胞株(RSC96)神经营养因子（NGF）及层粘连蛋白（Laminin， LN）的表达情况。方法 根据培养条件不同分3组，即平行静电纺丝培养组（A）、随机静电纺丝培养组（B）及膜上培养组（C）培养3天后用Trizol法提取细胞总RNA，用Oligo(dT)逆转录酶得到cDNA。经特定引物PCR扩增， 取相同量的逆转录产物进行qPCR反应, 检测NGF 及LN 的表达差异。结果 平行静电纺丝组，随机静电纺丝组，膜上培养组上NGF基因表达依次递增，各组间差异明显； LN基因的表达依次递减，各组间差异明显。 结论 不同空间排列的静电纺丝对RSC96细胞表达NGF和Laminin影响不同，平行的静电纺丝有促进细胞向成熟分化的可能。
曹欣 , 赵利 , 张贤祚 , 秦建强 , 李红 , 吴刚 . 不同空间排列静电纺丝对RSC96细胞表达NGF及Laminin的研究[J]. 中国临床解剖学杂志, 2015 , 33(1) : 60 -63 . DOI: 10.13418/j.issn.1001-165x.2015
Objective To study the expression of NGF and Laminin in RSC96 cells under different topography of eletrospun fibrous scaffolds. Methods According to different culture conditions, cultured RSC96 were divided into three groups, group A(aligned), group B(random) and group C(film), and all the three groups were cultured for three days. The total RNA of cells was extracted by Trizol method. Reverse transcriptase Oligo (dT) was used to synthesize cDNA. Then RNA was expanded by designed primer and used for q-PCR to detect the expression of NGF and Laminin. Results NGF mRNA expression of RSC96 was lower in group A , compared to group B and C (P＜0.05). There was statistical differences between group B and group C (P＜0.05). The laminin mRNA was expressed in all groups, but an decrease from group A to group C could be detected (P＜0.05). Conclusions The different expression of NGF and Laminin of RSC96 cells was induced by different topography of electrospun fibers and RSC96 cells may be directed towards a more mature state by aligned electrospun fibers.
Key words： RSC96 cells; NGF; Laminin; Electrospun fibers
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