三维多孔支架材料PLLA/n-HA的体外生物相容性研究
收稿日期: 2014-03-25
网络出版日期: 2015-02-12
基金资助
深圳市重点实验室提升项目（CXB201104220049A）；深圳市科技研发资金项目（CXZZ20120614160234842，JCYJ20130401113330839，CXZZ201303211 52713220）； 深圳市第二人民医院育苗计划
Three-dimensional porous scaffolds PLLA/n-HA in vitro biocompatibility studies
目的 研究三维多孔支架材料聚乳酸/纳米羟基磷灰石 (PLLA/n-HA)的体外生物相容性，探讨其作为细胞培养材料和骨组织工程支架的可行性。方法 将大鼠成骨细胞接种于PLLA/n-HA复合支架上，体外共同培养后，CCK-8法检测大鼠成骨细胞增殖活性，荧光倒置显微镜、扫描电子显微镜下观察PLLA/n-HA复合支架材料表面和孔隙内细胞粘附情况。结果 CCK-8法检测显示实验组复合支架材料上细胞的增殖与空白对照组的差异无统计学意义（P>0.05）；电镜观察到细胞在复合支架材料表面和孔隙内大量黏附、生长，并且随着共培养时间的增加，材料表面的细胞数量呈几何级增长。结论 三维多孔支架材料PLLA/n-HA的生物相容性较好，可望成为一种性能良好的骨组织工程支架材料。
黄江鸿 , 陈洁琳 , 段莉 , 张巨峰 , 熊建义 , 朱伟民 , 王大平 . 三维多孔支架材料PLLA/n-HA的体外生物相容性研究[J]. 中国临床解剖学杂志, 2015 , 33(1) : 55 -59 . DOI: 10.13418/j.issn.1001-165x.201
Objective To study the three-dimensional porous scaffolds of polylactic acid/nano- hydroxyapatite (PLLA/n-HA) in vitro biocompatibility, and to explore its suitability ascell culture materials and bone tissue engineering scaffolds. Methods The rat osteoblasts were seeded on PLLA/n-HA composite scaffolds after in vitro co-culture, CCK-8 assay was used to determine the rat osteoblast proliferation activity, inverted fluorescence microscope; scanning electron microscope were used to observe the PLLA/n- HA composite scaffold surface and the cell adhesion conditon within the pores. Results CCK-8 assay showed that the experimental group was not significantly different in proliferation of composite scaffolds cells with the control group (P>0.05); electron microscopy showed a large number of cell growth and adhesion in the composite scaffold surface and pores; and with the increase of culture time, the number of cells on material surface grew exponentially. Conclusion The three-dimensional porous scaffolds PLLA/n-HA have good biocompatibility and is expected to become a good performance of bone tissue engineering scaffolds.
Key words： Three-dimensional porous; PLLA; n-HA; Rat osteoblasts
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