不同剂量吗啡对C57小鼠成瘾行为学的影响
收稿日期: 2014-05-08
网络出版日期: 2014-12-04
基金资助
国家自然科学基金（81371509 )；教育部科学技术研究重点项目(21132)；粤港关键领域重点突破项目(2011A011304001)；广东省产学研合作重大科技专项(2011A090100025)；广州市科技计划项目(201300000183)；广东省高等学校人才引进项目(C2050205）。
Effects of different dosages of morphine on conditioned place preference in male C57 mice
Received date: 2014-05-08
Online published: 2014-12-04
目的 观察不同剂量的片剂吗啡作用后，C57小鼠的成瘾行为学变化，为吗啡成瘾的研究提供参考。 方法 用3月龄雄性C57小鼠分别腹腔注射0、5、10、15、20 mg/kg的吗啡，观察指标为竖尾反应持续的时间以及条件位置偏爱（Conditioned place preference ）中的白箱时间差。 结果 与对照组（0 mg/kg）相比，吗啡10、15和20 mg/kg组小鼠出现竖尾反应的时间有显著差异（P<0.05）；在条件位置偏爱实验中，与对照组相比，吗啡10、15、20 mg/kg 剂量组逆转了小鼠偏爱呆在黑箱的天然倾向，白箱时间差有统计学差异。其中，吗啡10 mg/kg组P=0.049，而15 mg/kg组和20 mg/kg组(P<0.001)。 结论 在吗啡剂量为10、15、20 mg/kg时，白箱偏爱明显形成，15 mg/kg是较为敏感而稳定的吗啡成瘾小鼠模型剂量，可作为敏感而又稳定的较低剂量。
谢敏娟 , 李娟 , 张璐 . 不同剂量吗啡对C57小鼠成瘾行为学的影响[J]. 中国临床解剖学杂志, 2014 , 32(6) : 708 -710 . DOI: 10.13418/j.issn.1001-165x.2014
Objective To observe the conditioned place preference (CPP) changes in mice after treated with different doses of morphine, and provide reference for the further study of morphine addiction. Method 3-month-old male C57 mice were injected intraperitoneally with 0 mg/kg, 5 mg/kg, 10 mg/kg, 15 mg/kg and 20 mg/kg of morphine, CPP and Straub tail reaction analysis were carried out. Result The Straub tail reaction analysis showed that: compared with control group, mice in morphine 10 mg/kg, 15 mg/kg, and 20 mg/kg groups demonstrated longer Straub tail reaction time (P< 0.05). The CPP analysis also showed that, compared with the control group mice，15mg/kg and 20mg/kg morphine reversed the natural tendency to black box of mice, which spent more time in the white box (P< 0.001). Meanwhile, the time spend in the white box was also statistically longer in the 10 mg/kg morphine group compared with the control group (P=0.049<0.05). Conclusion 15 mg/kg morphine can produce a significant adaptive change in CPP test in mice, which is an ideal dosage to construct morphine addiction model in mice.
Key words： C57 mice; Morphine; Dosage; Conditioned place preference; Straub tail reaction
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