NUCB2/Nesfatin-1 在DIO大鼠下丘脑中的表达
收稿日期: 2014-01-13
网络出版日期: 2014-12-04
基金资助
广西高等学校优秀人才资助计划项目（2012-43）；广西自治区级大学生创新创业训练计划资助项目（2012-586）
NUCB2/Nesfatin-1expression in the hypothalamus of DIO rats
Received date: 2014-01-13
Online published: 2014-12-04
目的 探讨NUCB2/Nesfatin-1在饮食诱导肥胖（DIO）大鼠下丘脑中的表达水平，为糖脂代谢神经调节机制的深入研究提供实验依据。 方法 通过高能饲料喂养建立高血糖高血脂大鼠模型，采用免疫组织化学方法检测下丘脑NUCB2/Nesfatin-1表达水平。 结果 ①NUCB2/Nesfatin-1在实验组大鼠下丘脑表达的阳性率为43.33%，明显低于对照组的80.00%（P<0.05）。② NUCB2/Nesfatin-1在实验组大鼠下丘脑的表达强度积分为2.159±0.918，对照组表达积分为3.683±1.250（P<0.01）。 结论 NUCB2/Nesfatin-1在饮食诱导肥胖（DIO）大鼠下丘脑的表达水平明显降低，提示NUCB2/Nesfatin-1可能在糖脂代谢神经调节中具有重要作用。
关键词： NUCB2/Nesfatin-1; 糖脂代谢; 下丘脑
秦小云 , 王伯钧 , 伍善广 , 张庆金 , 刘漫君 , 兰羚元 , 张梦雅 , 黄蓓 , 覃小华 , 夏春波 . NUCB2/Nesfatin-1 在DIO大鼠下丘脑中的表达[J]. 中国临床解剖学杂志, 2014 , 32(6) : 704 -707 . DOI: 10.13418/j.issn.1001-165x.2014
Objective To explore the NUCB2/Nesfatin-1 expression level in hypothalamus of diet induced obesity（DIO）rats, and to provide experimental basis for the mechanism of nerve regulation by glucolipid metabolism. Methods To setting up high glucose and lipid rat model by high-energy feed, using immunohistochemical method to detect the hypothalamus NUCB2/Nesfatin-1 expression level. Results （1）NUCB2/Nesfatin-1 expression positive rate was 43.33% in the hypothalamus of the experimental group, which was significantly lower than that（80.00%）in the control group（P<0.05）.（2）NUCB2/Nesfatin-1 expression strength score was 2.159±0.918 in the hypothalamus in the experimental group, and was 3.683±1.250 in the control group（P<0.01）. Conclusion NUCB2/Nesfatin-1 expression level was decreased obviously in the hypothalamus of diet induced obesity rats，suggesting NUCB2/Nesfatin-1 may play an important role in regulating glucolipid metabolism.
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