基于Amira5.4对小脑核团重建和体积测量的研究及其意义
收稿日期: 2014-05-17
网络出版日期: 2014-12-04
基金资助
天津医科大学重点学科项目资助（No.052-200010）
Reconstruction and volume measurement research and value of cerebellar nuclei based on Amira 5.4
Received date: 2014-05-17
Online published: 2014-12-04
目的 构建小脑各核团（deep cerebellar nuclei, DCN）的三维空间可视化模型并对其体积进行测量，以明确各小脑核团体积的男女性别差异，为小脑各核团的定位、临床功能及性别差异性研究提供参考数据。 方法 利用Amira 5.4对12组小脑火棉胶切片图像数据进行小脑核团的三维重建，并对各核团体积进行测量，比较各核团左、右侧及男女性体积差异。 结果 三维模型显示小脑各核团结构清楚，形态分布及其与第四脑室（fourth ventricle, FV）的空间关系亦能够清晰的展示。在左、右侧体积对比，各核团均无显著差异（P>0.05）；男、女性别对比，齿状核（dentate nucleus, DN）的体积男性远大于女性，差异有统计学意义（P<0.001），而另外3组核团的体积没有明显的男女性别差异（P>0.05）。 结论 对小脑核团的三维重建及体积测量可以明确各核团的形态、体积及其与第四脑室的空间关系，同时齿状核体积的男、女性别差异又为核团功能的进一步研究提供一定的参考价值。
张晓阳 , 王单单 , 袁武 , 李云生 . 基于Amira5.4对小脑核团重建和体积测量的研究及其意义[J]. 中国临床解剖学杂志, 2014 , 32(6) : 666 -670 . DOI: 10.13418/j.issn.1001-165x.2014
Objective To reconstruct the 3-D visualization model of cerebellar nuclei, and measure the volumes of them for defining the differences of the volumes of cerebellar nuclei between male and female, further providing reliable data for the researches about location, clinical function, and gender differences of cerebellar nuclei. Methods 12 groups of images were obtained from cerebellum specimens embedded by celloidin. Amira 5.4 was used to accomplish 3-D reconstruction and volume measurement of cerebellar nuclei, and then compared the volume of cerebellar nuclei between left and right, male and female. Results 3-D model showed that the structures and morphology of cerebellar nuclei were well-defined; the spatial relationship with the fourth ventricle was also displayed clearly. Regarding the volumes of cerebellar nuclei, we found no differences between the left and right by statistical comparison (P>0.05); a significant statistical difference was found in dentate nucleus between male and female (P<0.001), but for other cerebellar nuclei, there was no significant statistical difference (P>0.05). Conclusion The morphology, volumes of cerebellar nuclei and the spatial relationship with the fourth ventricle can be obtained explicitly by 3-D reconstruction; gender differences of the volumes of dentate nucleus also may provide important value for further function research of cerebellar nuclei.
Key words： Amira; Cerebellar nuclei; Volume measurement; Celloidin
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