尿道下裂尿道成形材料的Masson染色组织学特点
收稿日期: 2014-02-20
网络出版日期: 2014-06-04
Pathological features of urethral materials for urethroplasty
Received date: 2014-02-20
Online published: 2014-06-04
目的 通过观察尿道下裂尿道成形材料的组织学特点，指导手术材料的选择。 方法 收集2011年9月-2013年10月中山大学附属第一医院小儿外科重型尿道下裂患者阴茎阴囊皮肤标本共20例，每例均取包皮内板、阴茎体腹侧皮肤、阴囊中隔皮肤、阴囊周边皮肤四份标本，经福尔马林固定，石蜡包埋，超薄切片，Masson染色，置于高倍显微镜下由非本实验研究者利用正立显微成像系统随机拍取5个视野，由研究者观察并计算毛囊、皮脂腺、皮下血管密度以及测量皮肤厚度。 结果 毛囊及皮脂腺密度比较：阴囊中隔皮肤＜阴囊周边皮肤（P＜0.05），而包皮内板及阴茎体腹侧皮肤未见毛囊及皮脂腺。血管密度比较：包皮内板＞阴茎体腹侧皮肤＞阴囊中隔皮肤＞阴囊周边皮肤（各组比较P＜0.05）。皮肤厚度比较：包皮内板＜阴囊周边皮肤＜阴茎体腹侧皮肤＜阴囊中隔皮肤（各组比较P＜0.05）。 结论 包皮内板及阴茎体腹侧的皮肤是理想的尿道成形材料，材料不足时可适当使用阴囊中隔皮肤，阴囊周边皮肤一般不应用于尿道成形。
谢起根 , 苏诚 , 李作青 , 徐哲 , 孙俊杰 , 周李 . 尿道下裂尿道成形材料的Masson染色组织学特点[J]. 中国临床解剖学杂志, 2014 , 32(3) : 340 -342 . DOI: 10.13418/j.issn.1001-165x.2014
Objective To observe the pathological features of urethral materials for urethroplasty and provide guidance for the selection of urethroplasty materials. Methods From September 2011 to October 2013 in Pediatric Surgery of the First Affiliated Hospital of Sun Yat-sen University, 20 cases of proximal hypospadias were corrected for ventral curvature. During the operation, specimen including prepuce, ventral penile skin, scrotal septal skin and scrotal skin were collected, fixed and embedded in paraffin. Then tissues were cut into thin slices, followed by Masson staining and observation under the microscope. 5 HPF were photographed randomly by blinded researchers, then the density of hair follicles, sebaceous glands and vessel as well as the thickness of the skin were measured and calculated. Results Comparison of the density of hair follicles and sebaceous glands: scrotal septal skin < scrotal skin (P＜0.05), while no hair follicles and sebaceous glands were found in the prepuce and ventral penile skin. Comparison of vessel density: prepuce > ventral penile skin > scrotal septal skin > scrotal skin (P＜0.05). Comparison of skin thickness: prepuce < scrotal skin < ventral penile skin < scrotal septal skin (P＜0.05). Conclusions Prepuce and ventral penis skin are ideal candidates for urethroplasty, while the scrotal septal skin can be utilized when the Urethral material is insufficient. Scrotal skin is generally not recommended in urethroplasty.
Key words： Hypospadias; Urethral materials; Masson stainning
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