去细胞输尿管移植至膀胱壁后的再生
收稿日期: 2014-04-11
网络出版日期: 2014-06-04
基金资助
浙江省大学生科技创新活动计划“新苗人才计划”(2012R413004)；国家级大学生创新创业训练计划项目(201310343006，201210343008)，浙江省教育厅（NO.Y201327980）
The regeneration of an acellular ureter transplanted to the bladder
Received date: 2014-04-11
Online published: 2014-06-04
目的 通过去细胞技术，将带一部分膀胱壁的大鼠输尿管去细胞后，移植于另一只鼠膀胱壁上，在不重新种植细胞的情况下，利用机体作为“自体反应器”来重构支架，最终使输尿管再生。 方法 20只SD大鼠的输尿管经过Triton-x100和SDS去细胞后用去离子水漂洗并经过杀菌，支架经过形态学鉴定后移植入其他鼠中，行膀胱修补术同时远端结扎，经过1、2、3、4周获取移植物进行HE染色，免疫荧光法观察分析。 结果 移植输尿管被分成3个部分：粘附于膀胱的部分(B)、中间部分(M)、游离端(D)。发现4周后B部分有上皮细胞和平滑肌细胞，M部分有脂肪细胞，D部分被纤维化并且在4周后仍然无细胞。B部分在1~2周后，上皮细胞出现，4周后，平滑肌细胞出现。 结论 未播种输尿管在接合区域也能实现再生，并且先出现上皮层后出现肌层。
邵营宽 , 林刻智 , 余雅玲 , 饶志恒 , 李若冰 , 梅劲 . 去细胞输尿管移植至膀胱壁后的再生[J]. 中国临床解剖学杂志, 2014 , 32(3) : 330 -334 . DOI: 10.13418/j.issn.1001-165x.2014
Objective After decellularization, a rat’s ureter attached to a piece of bladder wall was transplanted to another rat’s bladder wall. Without reseeding any cells, the body itself was to reconstruct the ureter. Methods 20 SD rats’ ureter were decellularized by Triton-X100 and SDS followed by sterilization, after authentication of the structure of the scaffolds we transplanted it onto another rat’s bladder wall with ligation of the distal part of the ureter. After 1-4 weeks, we sacrificed those rats and processed the transplanted structures with HE stain and immunofluorescence for observation and analysis. Results We divided the ureters into three parts the part attached to the bladder(B)、the middle part(M)、the freeend (D). After 4 weeks , the epithelial cells and smooth muscles cells could be found in B part, fat cells in M part and fibrosis in D part. Particularly, after 1-2weeks, there are epithelial cells regrown in B part and muscle cells regrown after 4 weeks. Conclusion It is possible that ureter scaffolds can regenerate in body without reseeding and the epithelial layer would regrow first before muscular layer .
Key words： Tissue scaffolds; Regeneration; Ureter; Immunogenicity
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