中空羟基磷灰石复合rhBMP-2修复骨缺损过程的再血管化研究
收稿日期: 2013-10-28
网络出版日期: 2014-04-21
Revascularization study of the hollow hydroxyapatite composite rhBMP-2 in repairing the bone defect
Received date: 2013-10-28
Online published: 2014-04-21
目的 探讨和观察中空羟基磷灰石复合rhBMP-2在骨缺损修复过程的再血管化。 方法 将48只成年的新西兰雄性大白兔制作成桡骨骨缺损模型，随机分3组，各组分别植入以下材料：中空HA/ rhBMP-2复合人工骨、单纯中空HA人工骨、单纯rhBMP-2。植入后于4、8、12、16周分别注射99mTc-MDP进行放射性核素骨显像并监测骨缺损修复过程中再血管化情况，同时进行大体、X线、组织学观察。 结果 术后各时间段，中空HA/ rhBMP-2复合人工骨组在X线及放射性核素聚集强度明显高于单纯中空HA人工骨组(P<0.05) ，表现为成骨代谢活跃及早期的再血管化能力。 结论 中空HA/ rhBMP-2复合人工骨具有良好的骨缺损修复能力，成骨活性持久，再血管化能力强，有望成为一种理想的骨缺损修复材料。
关键词： 重组人骨诱导蛋白-2; 中空羟基磷灰石; 骨缺损; 再血管化
罗鹏刚 , 熊龙 , 曾建华 , 熊浩 , 吴增志 , 凌华军 , 夏雄超 . 中空羟基磷灰石复合rhBMP-2修复骨缺损过程的再血管化研究[J]. 中国临床解剖学杂志, 2014 , 32(2) : 196 -200 . DOI: 10.13418/j.issn.1001-165x.2014
Objective To study and observe the revascularization process of the hollow hydroxyapatite composite rhBMP-2 in repairing the bone defect. Methods 48 adult male New Zealand rabbits with artificially-established radius defect were randomly divided into 3 groups, each group were implanted with the following materials: hollow HA/ rhBMP-2 composite artificial bone(A groups), hollow HA(B groups),rhBMP-2(C groups). After implantation in 4、8、12、16 weeks, the process was observed using radionuclide bone imaging, X ray, histological observation. Results Different time after the operation, X-ray and histological score, bone radionuclide-gathering strength of the rabbits in the hollow HA/ rhBMP-2 composite artificial bone group in the were higher than that of hollow HA artificial bone (P＜0.05). In the control group, synostosis and bone sclerosis were observed in bone defect area bone defect failed to heal. Conclusion Hollow HA/ rhBMP-2 composite artificial bone has good ability in revascularization and repairing segmental bone defect and is expected to be an ideal material for bone defect repair.
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