实时超声在介导评估可注射CS/nHAC骨支架材料中的应用
收稿日期: 2013-05-15
网络出版日期: 2014-04-21
基金资助
广东省自然科学基金（10151051501000107）；深圳市战略新星产业发展专业资金项目（CXZZ20120618115626322）
A novel quantitative ultrasound technique for osteogenesis evaluation of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold
Received date: 2013-05-15
Online published: 2014-04-21
目的 探讨实时超声在介导可注射骨材料的注入过程和评估其原位成骨情况中的应用价值。 方法 将可注射性壳聚糖/纳米羟基磷灰石/胶原（CS/nHAC）支架材料注入大鼠皮下组织，分别于注射后1、14、28 d进行超声成像检查，定量测定支架的灰度值，半定量监测支架的体积及内部血供情况。 结果 在实时超声的监测下，支架材料准确注入骨缺损部位，并且在通过超声对材料的体内评估中发现，与壳聚糖/胶原（CS）支架相比，CS/nHAC支架表现出较大的硬度，较稳定的体内降解率及较好的血供。 结论 实时超声可直观、安全、准确地介导骨支架材料的注入过程，并能无创地评估可注射性骨支架材料在体内的固化过程、降解过程和血供情况，在评估可注射性骨材料方面有较好的应用前景。
阎禹廷 , 李鹤 , 袁月 , 李松建 , 陈彦 . 实时超声在介导评估可注射CS/nHAC骨支架材料中的应用[J]. 中国临床解剖学杂志, 2014 , 32(2) : 162 -166 . DOI: 10.13418/j.issn.1001-165x.2014
Objective An injectable scaffold based on nano-hydroxyapatite (nHA), collagen (Col) and chitosan (CS) was synthesized in a preliminary study. In this study, we proposed a novel quantitative noninvasive technique for osteogenesis evaluation of the scaffold. Methods The CS and CS/nHAC scaffold was respectively subcutaneously injected into Sprague-Dawley rats mediated by ultrasound. After 1h, 14d and 28d, the gray-scale value, volume and blood flow of the scaffold were measured using diagnostic technique software. The animals were evaluated by type-B ultrasonic and color doppler ultrasonography. Results Under mediation of ultrasound, the scaffold was accurately injected in. With evaluation of 28 days, we found the ROI gray-value of CS/nHAC was increasingly faster than CS/nHAC and the volume of CS/nHAC reduced slower. Conclusions CS/nHAC is more appropriate for fracture repairing than CS. And the ultrasound is proved a novel noninvasive technique for osteogenesis evaluation.
Key words： Injectable scaffold; Ultrasound; Noninvasive
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