目的 研究骨质疏松对小鼠骨髓间充质干细胞（mBMSCs）增殖、分化的影响，为骨质疏松的治疗提供理论和实验支持。 方法 30只C57BL/6小鼠随机分为假手术组和手术组，假手术组只去除卵巢周围脂肪，手术组去除卵巢。手术后8周分别取小鼠股骨和胫骨，分离培养mBMSCs，观察其增殖、分化的变化。 结果 骨质疏松小鼠的mBMSCs的增殖、迁移能力下降。碱性磷酸酶染色结果显示骨质疏松小鼠的mBMSCs成骨分化能力下降。 结论 骨质疏松模型小鼠mBMSCs的增殖、迁移和分化能力下降。
关键词：
骨质疏松; ; ; 小鼠骨髓间充质干细胞（mBMSCs）; ; ; 增殖; ; ; 迁移; ; ; 分化
Objective To study the effect of osteoporosis on the proliferation and differentiation of mouse bone marrow mesenchymal stem cells (mBMSCs), and to provide theoretical and experimental support for the treatment of osteoporosis. Methods Thirty C57BL/6 mice were randomly divided into sham operation group and operation group. Sham operation group only removed the fat around the ovary, and operation group removed the ovary. mBMSCs were taken from the femur and tibia of mice and cultured 8 weeks after operation to observe the changes of proliferation and differentiation. Results The proliferation and migration ability of mBMSCs in osteoporosis mice were decreased. The results of alkaline phosphatase staining showed that mBMSCs had decreased osteogenic differentiation ability in osteoporosis mice. Conclusions The proliferation, migration, and differentiation ability of mBMSCs in osteoporotic mice were decreased.
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