推拿扌衮法对骨骼肌钝挫伤家兔模型损伤后炎症及纤维化的影响

阮磊, 黄博, 王兰兰, 薛惠天, 孙梦龙, 段苗苗, 彭亮

doi:10.13418/j.issn.1001-165x.2024.2.13

中国临床解剖学杂志 >

2024 , Vol. 42 >Issue 2: 191 - 195

DOI: https://doi.org/10.13418/j.issn.1001-165x.2024.2.13

实验研究

推拿扌衮法对骨骼肌钝挫伤家兔模型损伤后炎症及纤维化的影响

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湖南中医药大学针灸推拿与康复学院，长沙 410208

阮磊（1998-），女，硕士研究生，研究方向：推拿治病机理，E-mail：2969015517@qq.com

彭亮，教授，博士研究生导师，E-mail：550639573@qq.com

收稿日期: 2023-04-06

网络出版日期: 2024-04-22

基金资助

国家自然科学基金面上项目（82174521）

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The effect of tui na rolling on post-injury inflammation and fibrosis in rabbits in a model of blunt contusion of skeletal muscles

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College of Acupuncture, Massage and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China

Received date: 2023-04-06

Online published: 2024-04-22

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摘要

目的探讨推拿扌衮法对家兔骨骼肌钝挫伤后相关纤维蛋白表达的影响，以及对骨骼肌钝挫伤修复的作用机制。方法健康成年新西兰兔15只，随机分为空白组（A）、模型组（B）和治疗（C）组，每组5只。使用自制改良重力锤打击装置对模型组和治疗组建立骨骼肌钝挫伤模型，治疗组家兔于造模成功后7 d给予推拿扌衮法干预，频率140次/min，2次/d，3 min/次，共治疗3 d。干预结束后1 d进行取材。HE和Masson染色观察兔股四头肌病理改变；Western-blot检测各组股四头肌基质金属蛋白酶-9（matrix metalloproteinases-9，MMP-9）、特异性抑制剂-1（tissue inhibitor of metalloproteinases-1，TIMP-1）、转化生长因子-β1（transforming growth factor-β1，TGF-β1）及I型胶原蛋白（I collagen，COL-I）表达。结果空白组肌组织结构规整；模型组肌组织形态迥异，边界模糊，间隙显著增宽，炎细胞浸润显著，四周胶原纤维生成增加；治疗组肌组织结构相对完整，间隙缩小，可见少量炎细胞浸润与胶原纤维生成，其病理修复程度显著优于模型组。检测家兔股四头肌MMP-9、TIMP-1、TGF-β1、COL-I表达，与空白组相比，模型组与治疗组明显增加（P<0.01）；但治疗组显著低于模型组（P<0.01）。结论推拿扌衮法能够通过减少细胞外基质过度沉积抑制骨骼肌纤维化，其作用机制可能与下调MMP-9、TIMP-1、TGF-β1及COL-I表达量有关。

关键词： 推拿扌衮 ; 法； ; ; 骨骼肌钝挫伤； ; ; 基质金属蛋白酶-9； ; ; 基质金属蛋白酶抑制剂-1； ; ; 转化生长因子-β1； ; ; I型胶原蛋白

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阮磊, 黄博, 王兰兰, 薛惠天, 孙梦龙, 段苗苗, 彭亮 . 推拿扌衮法对骨骼肌钝挫伤家兔模型损伤后炎症及纤维化的影响[J]. 中国临床解剖学杂志, 2024 , 42(2) : 191 -195 . DOI: 10.13418/j.issn.1001-165x.2024.2.13

Abstract

Objective To investigate the effect of tui na rolling method on the expression of relevant fibrosis proteins after blunt contusion injury of skeletal muscle in rabbits, and to explore the mechanism of action of tui na rolling method on the repair of blunt contusion injury of skeletal muscle. Methods Fifteen healthy adult New Zealand rabbits were randomly divided into three groups of blank (A), model (B) and treatment (C), with five rabbits in each group. Blunt contusion of skeletal muscle was modelled in rabbits of groups B and C using a self-constructed modified gravity hammer percussion device. The rabbits in group C were given the intervention 7 d of tui na rolling after successful modelling, at a frequency of 140 beats/min, 2 times/d, 3 min/session, for a total of 3 d of treatment. Tissue sampling was performed 1 d after the end of the intervention. Histological changes of rabbit quadriceps femoris muscle were observed by HE and Masson staining, and MMP-9, TIMP-1, TGF-β1, and COL-I expression of quadriceps femoris muscle of rabbits in each group were detected by Western-Blot. Results The muscle tissue of the blank group was of regular structure, the muscle tissue of the model group was of very distinct shape, with blurred margins, significantly enlarged gaps, significant invasion of inflammatory cells, and increased collagen fiber production in the surrounding area, the muscle tissue of the treatment group was relatively more intact in structure, with the gaps narrowed, and a small amount of invasion of inflammatory cells and collagen fiber production could be seen, and the degree of its pathological repair was significantly better than that of the model group. Compared with the blank group, the expression of MMP-9, TIMP-1, TGF-β1, and COL-I in quadriceps femoris muscle of rabbits in the model group and the treatment group were significantly increased (P<0.01). However, the expression of MMP-9, TIMP-1, TGF-β1, and COL-I in quadriceps femoris muscle of rabbits in the treatment group was significantly lower than that in the model group (P<0.01). Conclusions Tui na rolling method is ability to inhibit skeletal muscle fibrosis by reducing the excessive deposition of extracellular matrix, and then inhibit skeletal muscle fibrosis, and its mechanism of action maybe related to the suppression of the expression of MMP-9, TIMP-1, TGF-β1, and COL-I.

Key words： Tui Na rolling; ; Skeletal muscle blunt contusion; ; Matrix metalloproteinase-9; ; Matrix metalloproteinase inhibitor-1; ; ; Transforming growth factor-β1; ; ; Type I collagen

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